Diphtheria Toxin from Corynebacterium diphtheriae

lyophilized powder, Product is in unnicked form

MDL number:

Quality Level


lyophilized powder

storage temp.


Looking for similar products? Visit Product Comparison Guide


Diphtheria toxin may be used as a toxin element in the construction of immunotoxin for cell specific cytotoxicity. The translocation domain of bacterial toxins with natural endosome escape mechanism has been used in the development of efficient nonviral vectors for applications in gene therapy.

Biochem/physiol Actions

Inhibits protein synthesis by catalyzing ADP-ribosylation of eukaryotic aminoacyltransferase II.

Physical form

Lyophilized powder containing Tris and EDTA.


Each vial, when reconstituted to 0.5 mL with sterile distilled water, contains ~1 mg of diphtheria toxin in 0.01 M Tris and 0.001 M Na2EDTA, pH 7.5.


Skull and crossbones

Signal Word


Hazard Statements


Acute Tox. 1 Inhalation - Acute Tox. 1 Oral


6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the solubility of Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae?

    This product is soluble in water.  Reconstitute with the volume of water described under Description on our website page for D0564.  

  4. What is the solution stability of Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae?

    Once the product is reconstituted in water, the solution may be stored in aliquots at -20°C. Avoid freeze-thawing. Long-term storage in the refrigerator at 2-8°C is not recommended.

  5. How stable is Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae, at low concentration?

    For assays employing low concentration of the toxin, the use of a carrier protein like serum albumin is recommended.   

  6. What is the molecular weight of Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae?

    In the presence of a reducing agent, on SDS gels, this preparation migrates as a major band of approx. mw 63,000 Daltons (corresponding to thte intact toxin) and two faster more lightly stained bands of approx. MW 39,000 and 24,000 Daltons (corresponding to the B and A fragments, respectively). Based on this method, the extent of the degree of nicking in this preparation is estimated to be less than 5%.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. How is Product D0564-1MG, Diphtheria Toxin, packaged?

    Product D0564-1MG, Diphtheria Toxin, is packaged in a small serum vial with a rubber or silicone septum and aluminum crimp top. The product can be reconstituted in the vial by piercing the septum with a syringe fitted with a stainless steel needle.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

S M Yellon et al.
Biology of reproduction, 100(5), 1386-1394 (2019-01-11)
To test the hypothesis that macrophages are essential for remodeling the cervix in preparation for birth, pregnant homozygous CD11b-dtr mice were injected with diphtheria toxin (DT) on days 14 and 16 postbreeding. On day 15 postbreeding, macrophages (F4/80+) were depleted...
Mary F Fontana et al.
PLoS pathogens, 12(12), e1006046-e1006046 (2016-12-07)
Dynamic regulation of leukocyte population size and activation state is crucial for an effective immune response. In malaria, Plasmodium parasites elicit robust host expansion of macrophages and monocytes, but the underlying mechanisms remain unclear. Here we show that myeloid expansion...
Benjamin J Vakoc et al.
Nature medicine, 15(10), 1219-1223 (2009-09-15)
Intravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the ability...
David Bauché et al.
Immunity, 49(2), 342-352 (2018-08-12)
Interleukin-22 (IL-22)-producing group 3 innate lymphoid cells (ILC3) maintains gut homeostasis but can also promote inflammatory bowel disease (IBD). The regulation of ILC3-dependent colitis remains to be elucidated. Here we show that Foxp3+ regulatory T cells (Treg cells) prevented ILC3-mediated colitis in...
Allah Nawaz et al.
Nature communications, 8(1), 286-286 (2017-08-19)
Adipose tissue resident macrophages have important roles in the maintenance of tissue homeostasis and regulate insulin sensitivity for example by secreting pro-inflammatory or anti-inflammatory cytokines. Here, we show that M2-like macrophages in adipose tissue regulate systemic glucose homeostasis by inhibiting...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service