MilliporeSigma
All Photos(4)

D1033

Sigma-Aldrich

Monoclonal Anti-Desmin antibody produced in mouse

clone DE-U-10, ascites fluid

Synonym(s):
Anti-LGMD2R, Anti-CDCD3, Anti-CSM1, Anti-LGMD1D, Anti-CSM2, Anti-LGMD1E
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DE-U-10, monoclonal

mol wt

antigen 50-55 kDa

contains

15 mM sodium azide

species reactivity

feline, sheep, bovine, hamster, chicken, viper, rat, rabbit, lizard, mouse, human, goat

technique(s)

indirect immunofluorescence: 1:40 using formalin-fixed, paraffin-embedded sections of human tissue
microarray: suitable
western blot: 1:200 using chicken gizzard extract/whole extract of mouse myoblast C2C12 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Des(13346)
rat ... Des(64362)

General description

Desmin is the protein subunit of muscle-type intermediate filaments and is found predominantly in skeletal, cardiac, and smooth muscle.
Monoclonal Anti-Desmin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The desmin protein contains a head domain, four α-helical segments, three non-helical rod segments and a tail domain. It exists as dimer and tetramer assemblies. Desmin is is present in the periphery of Z-disk in smooth and striated muscles.

Specificity

The antibody localizes desmin in cultured cells and tissue sections from several species and in tumors derived from muscle tissue (leiomyomas, rhabdomyosarcomas).

Immunogen

desmin from pig stomach

Application

Monoclonal Anti-Desmin antibody produced in mouse has been used in:
  • immunohistochemical staining
  • immunoblot analyses
  • immunocytochemistry
  • immunocytochemical localization of intermediate filaments

Monoclonal Anti-Desmin antibody produced in mouse is suitable for indirect immunofluorescence at a working dilution of 1:20 using formalin-fixed, paraffin-embedded sections of human tissue, microarray and western blotting. It may be used for immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs. It specifically stains desmin when used in immunoblotting assays. It may be used for labelling desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas). It was used for immunohistochemical staining of cardiac cells in a study to understand cardiac conduction through engineered tissue. It was used as a primary antibody for the detection of expression of desmin in cultured cells to study the effect of mutation in desmin gene.

Biochem/physiol Actions

Desmin provides mechanical strength to muscle tissues. Mutations in desmin gene contributes to imbalance in skeletal proteins, resulting in desminopathies. Mutations in this gene damages the stability of intermediary filaments in cardiomyocytes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Quotes and Ordering

Functional characterization of the novel DES mutation p. L136P associated with dilated cardiomyopathy reveals a dominant filament assembly defect
Brodehl A, et al.
Journal of Molecular and Cellular Cardiology, 91, 207-214 (2016)
K Rosenvold et al.
Journal of animal science, 88(5), 1830-1841 (2010-02-02)
The current pork slaughter process is primarily optimized to reduce cooler shrink and the incidence of PSE pork. Elimination of the halothane gene and improved preslaughter handling have decreased the incidence of PSE pork and improved the water-holding capacity of
Andreas Brodehl et al.
Circulation. Cardiovascular genetics, 6(6), 615-623 (2013-11-10)
The intermediate filament protein desmin is encoded by the gene DES and contributes to the mechanical stabilization of the striated muscle sarcomere and cell contacts within the cardiac intercalated disk. DES mutations cause severe skeletal and cardiac muscle diseases with
Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration
Park S, et al.
International Journal of Molecular Medicine, 37(5), 1209-1220 (2016)
Phenotypic expression of a novel desmin gene mutation: hypertrophic cardiomyopathy followed by systemic myopathy.
Harada H, et al.
Journal of Human Genetics, 63(2), 249-249 (2018)

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