D1313

Sigma-Aldrich

JumpStart REDAccuTaq® LA DNA Polymerase

Long and accurate hot-start Taq with inert dye, 10X buffer included

NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

form

liquid

feature

hotstart

concentration

1 unit/μL

color

red

Featured Industry

Agriculture

shipped in

wet ice

storage temp.

−20°C

Related Categories

Application

JumpStart REDAccuTaq® LA DNA Polymerase has been used for amplifying genomic DNA.
JumpStart REDAccuTaq LA DNA Polymerase is a unique enzyme blend that is capable of generating long PCR fragments, from 0.25 kb to 40 kb, with high fidelity, increased specificity and yield. JumpStart REDAccuTaq DNA polymerase combines Sigma′s AccuTaq LA DNA polymerase and JumpStart Taq antibody with an inert red dye. This specially formulated hot start enzyme mix achieves greater yields, enhances sensitivity and results in higher fidelity (6.5×) in comparison to standard Taq or other Long and Accurate enzyme blends. Its high fidelity makes it the enzyme of choice when performing amplifications where a low error frequency is critical, such as in RT-PCR and cloning.

JumpStart REDAccuTaq LA DNA Polymerase also contains the hot start mechanism of the JumpStart Taq antibody. JumpStart Taq antibody is designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq antibody does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction.

The inert red dye provides quick recognition and confirmation of appropriate mixing. An aliquot of the samples (5-10 μL) may be loaded directly onto an agarose gel following PCR. The red dye migrates slightly faster than bromophenol blue at the same rate as a 125 base pair fragment.

The PCR product can be easily separated from the dye by standard purification methods. The inert red dye has does not effect automated sequencing, restriction enzyme digestion, ligation or other downstream applications.

Features and Benefits

  • JumpStart REDAccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
  • Up to 6.5X greater fidelity in comparison to Taq DNA polymerase making it the ideal enzyme for multiplex PCR
  • Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
  • Reduce set-up time and eliminate concerns associated with manual or wax Hot Start methods
  • Dye allows for quick visual confirmation that reagent has been added and mixed properly
  • Direct loading onto an agarose gel without additional dyes

Packaging

Supplied with optimized 10× reaction buffer

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min at 74 °C.

Other Notes

View more detailed information on JumpStart REDTaq and Accutaq enzymes at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDAccuTaq is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Roberto Giorda et al.
American journal of human genetics, 85(3), 394-400 (2009-09-01)
Submicroscopic copy-number variations make a considerable contribution to the genetic etiology of human disease. We have analyzed subjects with idiopathic mental retardation (MR) by using whole-genome oligonucleotide-based array comparative genomic hybridization (aCGH) and identified familial and de novo recurrent Xp11.22-p11.23...
Yukiko Yamazaki et al.
Molecular reproduction and development, 73(2), 180-188 (2005-10-26)
During differentiation, somatic cell nuclei acquire unique patterns of epigenetic modifications, such as DNA methylation, which affect the transcriptional activity of specific genes. Upon transfer into oocytes, however, the somatic nucleus undergoes reprogramming of these epigenetic modifications to achieve pluripotency....
Single loci detection and karyotyping using small target FISH on maize somatic chromosomes
Lamb J C, et al.
Genetics (2007)
Mark Sharkey et al.
Journal of virology, 79(8), 5203-5210 (2005-03-30)
Current regimens for the management of human immunodeficiency virus type 1 (HIV-1) infection suppress plasma viremia to below detectable levels for prolonged intervals. Nevertheless, there is a rapid resumption in plasma viremia if therapy is interrupted. Attempts to characterize the...
Scott T Lefurgy et al.
Protein science : a publication of the Protein Society, 16(12), 2636-2646 (2007-11-22)
In class C beta-lactamases, the strictly conserved Asn152 forms part of an extended active-site hydrogen-bonding network. To probe its role in catalysis, all 19 mutants of Enterobacter cloacae P99 cephalosporinase Asn152 were simultaneously constructed and screened in Escherichia coli for...
Articles
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
Read More
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Read More
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Read More
Protocols
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Read More
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Read More

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