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D1408

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

10×, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
DPBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
glucose: no (Dextro)

shipped in

ambient

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This Item
59331C59321CD1283
form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

-

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

DPBS, Isotonic saline, cell washing solution, cell suspension solution

Isotonic saline, cell washing solution, cell suspension solution

Isotonic saline, cell washing solution, cell suspension solution

DPBS, Isotonic saline, cell washing solution, cell suspension solution

General description

Dulbecco′s Phosphate Buffered Saline (DPBS) is a balanced salt solution.

Application

Dulbecco′s Phosphate Buffered Saline has been used:
  • in microfluidic cell culture of mouse embryonic stem cells (mESC)
  • to dissolve albumin for adsorption studies
  • in PC12 cell culture experiments

Dulbecco′s Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water (W3500). D-PBS is used to wash cells during preparation and serial transfer.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sarah M Hicks et al.
The Journal of infectious diseases, 223(1), 10-14 (2020-10-04)
Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike
Marjan Nabili et al.
Ultrasound in medicine & biology, 39(4), 638-646 (2013-02-19)
Delivery of sufficient amounts of therapeutic drugs into the eye is often a challenging task. In this study, ultrasound application (frequencies of 400 KHz to 1 MHz, intensities of 0.3-1.0 W/cm(2) and exposure duration of 5 min) was investigated to overcome
Alexandre Super et al.
Biotechnology journal, 11(9), 1179-1189 (2016-05-24)
Oxygen plays a key role in stem cell biology as a signaling molecule and as an indicator of cell energy metabolism. Quantification of cellular oxygen kinetics, i.e. the determination of specific oxygen uptake rates (sOURs), is routinely used to understand
A D Cabral et al.
The Analyst, 142(20), 3922-3933 (2017-09-21)
Chemosensors for the detection of phosphate-containing biological species are in high need. Detection of proximally phosphorylated sites of PP
Yuta Tanaka et al.
Journal of biomedical materials research. Part A, 92(1), 350-358 (2009-02-04)
Both terminals of a poly(ethylene glycol) (PEG) molecule were terminated with amines. The PEG was electrodeposited onto titanium (Ti) to give it the biofunctions such as blood compatibility. The effects of the amine of PEG terminals and the pH at

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