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Key Documents

D1501

Sigma-Aldrich

Deoxyribonucleic acid sodium salt from calf thymus

Type I, fibers

Synonym(s):

ctDNA, DNA sodium salt from calf thymus, Thymonucleic acid sodium salt

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51

type

Type I

form

fibers

color

white

storage temp.

2-8°C

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Application

Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.

Quality

"Highly Polymerized"

Physical properties

λmax 259 nm (100 mM phosphate buffer, pH 7.0).
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.

Physical form

fibrous preparation

Preparation Note

This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.
This product is prepared from calf thymus tissue of unspecified gender.

Reconstitution

Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (μg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.
This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Oskar Szczepaniak et al.
Biomolecules, 10(5) (2020-05-06)
Since ancient times, fruits and edible plants have played a special role in the human diet for enhancing health and maintaining youthfulness. The aim of our work was to determine the interactions between naringin, a natural ingredient of grapefruits, and
A M Parker et al.
Journal of dairy science, 99(12), 9875-9884 (2016-10-04)
Bacterial contamination of milk fed to calves compromises calf health. Several bacterial pathogens that infect cows, including Mycoplasma bovis and Salmonella enterica ssp. enterica serovar Dublin, are shed in milk, providing a possible route of transmission to calves. Milk acidification
Hironobu Ikehata et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 17(4), 404-413 (2018-02-22)
The amount of photolesions produced in DNA after exposure to physiological doses of ultraviolet radiation (UVR) can be estimated with high sensitivity and at low cost through an immunological assay, ELISA, which, however, provides only a relative estimate that cannot
M S Hazelton et al.
Journal of dairy science, 103(12), 11844-11856 (2020-09-29)
Replacement dairy heifers exposed to Mycoplasma bovis as calves may be at risk of future clinical disease and pathogen transmission, both within and between herds; however, little information is available about these risks. We conducted a 2-yr longitudinal (panel) study
Fariba Mollarasouli et al.
Journal of pharmaceutical analysis, 10(5), 473-481 (2020-11-03)
In this study, an electrochemical DNA biosensor was developed using a straightforward methodology to investigate the interaction of indinavir with calf thymus double-stranded deoxyribonucleic acid (ct-dsDNA) for the first time. The decrease in the oxidation signals of deoxyguanosine (dGuo) and

Protocols

Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.

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