MilliporeSigma
All Photos(1)

Documents

D1529

Sigma-Aldrich

1-Fluoro-2,4-dinitrobenzene

≥99%

Sign Into View Organizational & Contract Pricing

Synonym(s):
2,4-Dinitro-1-fluorobenzene, DNFB, DNPF, FDNB, Sanger reagent
Linear Formula:
(O2N)2C6H3F
CAS Number:
Molecular Weight:
186.10
Beilstein/REAXYS Number:
398632
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

assay

≥99%

form

solid

refractive index

n20/D 1.569 (lit.)

bp

178 °C/25 mmHg (lit.)

mp

25-27 °C (lit.)

density

1.482 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

[O-][N+](=O)c1ccc(F)c(c1)[N+]([O-])=O

InChI

1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H

InChI key

LOTKRQAVGJMPNV-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
4208573177D102504
vibrant-m

D1529

1-Fluoro-2,4-dinitrobenzene

vibrant-m

42085

1-Fluoro-2,4-dinitrobenzene

vibrant-m

73177

1-Fluoro-2,4-dinitrobenzene

vibrant-m

D102504

1,5-Difluoro-2,4-dinitrobenzene

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

form

solid

form

liquid

form

(Solid or Semi-Solid or Chunk(s) or Paste or Liquid)

form

powder

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-

storage temp.

-

refractive index

n20/D 1.569 (lit.)

refractive index

n20/D 1.569 (lit.)

refractive index

n20/D 1.569 (lit.)

refractive index

-

bp

178 °C/25 mmHg (lit.)

bp

178 °C/25 mmHg (lit.)

bp

178 °C/25 mmHg (lit.)

bp

-

General description

1-Fluoro-2,4-dinitrobenzene (DNFB) is a reagent that modifies the N-terminal amino acids of proteins and peptides. DNFB is also commonly known as Sanger′s Reagent, named after Frederick Sanger, who reported in a classic 1945 paper the use of DNFB for derivatization of proteins and peptides specifically at their N-termini.†

Application

1-Fluoro-2,4-dinitrobenzene has been used to induce severe contact hypersensitivity in mice and to monitor gene expression by in vivo imaging method, and to induce chronic itch as a model for analysing itch-scratching behavior.

Biochem/physiol Actions

1-Fluoro-2,4-dinitrobenzene is a contact-sensitizing hapten, commonly used in experimental studies on contact hypersensitivity, inducing itch model, and atopic dermatitis. Mast cell activation is a key step in the DNFB induced sensitivity. DNFB covalently binds to the N-terminal amino acid of the protein and aids in Sanger sequencing.

pictograms

Exclamation markHealth hazard

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

327.2 °F

flash_point_c

164 °C

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 3

1 of 3

Metformin impurity F European Pharmacopoeia (EP) Reference Standard

Y0001600

Metformin impurity F

Tobramycin United States Pharmacopeia (USP) Reference Standard

USP

1667508

Tobramycin

Brian C Palmer et al.
HSOA journal of toxicology : current research, 4(1) (2020-11-10)
Titanium dioxide (TiO2) nanoparticles are commonly found in consumer products, such as sunscreens, and human dermal exposures are relatively high. Research suggests potential differences in the toxicity of anatase and rutile crystalline forms of TiO2. Additionally, transition metal dopants are
Bamboo salt suppresses skin inflammation in mice with 2, 4-dinitrofluorobenzene-induced atopic dermatitis
Yoou MS, et al.
Chinese Journal of Natural Medicines, 16(2), 97-104 (2018)
Beatrice Parma et al.
Journal of experimental & clinical cancer research : CR, 40(1), 248-248 (2021-08-09)
The identification of novel targets is of paramount importance to develop more effective drugs and improve the treatment of non-small cell lung cancer (NSCLC), the leading cause of cancer-related deaths worldwide. Since cells alter their metabolic rewiring during tumorigenesis and
Tac1-Expressing Neurons in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation
Gao ZR, et al.
Neuron, 101(9), 45-59 (2018)
Microbial Genetics (2014)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service