D1806

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer containing MgCl₂

• Synonym(s): MgCl₂ taq polymerase, Taq polymerase
• CAS Number: 9012-90-2
• Enzyme Commission number: 2.7.7.7 (BRENDA, IUBMB)
• MDL number: MFCD00166026
• NACRES: NA.55

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: liquid
• usage: sufficient for 10000 reactions; sufficient for 3000 reactions; sufficient for 500 reactions
• mol wt: 94 kDa
• feature: dNTPs included: no; hotstart: no
• concentration: 5 units/μL
• technique(s): PCR: suitable
• color: colorless
• input: purified DNA
• suitability: suitable for PCR and automated sequencing reactions
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It can withstand repeated heating to 95 °C without significant loss of activity. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing.

Application

Taq DNA Polymerase from Thermus aquaticus has been used:

• in the quantification of fungal growth by polymerase chain reaction (PCR) and photometric assay
• in conventional reverse transcriptase (RT)-PCR
• in simple sequence repeats (SSR) genotyping
• as a component of PCR mix for amplification of genomic and mitochondrial DNA
• in direct tetra-primer amplification refractory mutation system (T-ARMS) PCR to amplify dried whole blood samples

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

• Low per unit cost of Taq

Packaging

Taq DNA Polymerase with 10× reaction buffer containing MgCl₂

Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl₂ (D1806) or a 10× reaction buffer without MgCl₂ plus a separate tube of MgCl₂ for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Other Notes

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3