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D1806

Sigma-Aldrich

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer containing MgCl2

Synonym(s):

MgCl2 taq polymerase, Taq polymerase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.55

biological source

enzyme from bacterial (Thermus Aquaticus)

Quality Level

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 10000 reactions
sufficient for 3000 reactions
sufficient for 500 reactions

mol wt

94 kDa

feature

dNTPs included: no
hotstart: no

concentration

5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR and automated sequencing reactions

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It can withstand repeated heating to 95 °C without significant loss of activity. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing.

Application

Taq DNA Polymerase from Thermus aquaticus has been used:
  • in the quantification of fungal growth by polymerase chain reaction (PCR) and photometric assay
  • in conventional reverse transcriptase (RT)-PCR
  • in simple sequence repeats (SSR) genotyping
  • as a component of PCR mix for amplification of genomic and mitochondrial DNA
  • in direct tetra-primer amplification refractory mutation system (T-ARMS) PCR to amplify dried whole blood samples

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

  • Low per unit cost of Taq

Packaging

Taq DNA Polymerase with 10× reaction buffer containing MgCl2
Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Other Notes

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Severine Hurni et al.
The Plant journal : for cell and molecular biology, 76(6), 957-969 (2013-10-16)
The improvement of wheat through breeding has relied strongly on the use of genetic material from related wild and domesticated grass species. The 1RS chromosome arm from rye was introgressed into wheat and crossed into many wheat lines, as it
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Sonmez Z, et al.
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Fundamental study on the influence of Fusarium infection on quality and ultrastructure of barley malt
Oliveira P M, et al.
International Journal of Food Microbiology, 156(1), 32-43 (2012)
Lactic acid bacteria bioprotection applied to the malting process. Part II: Substrate impact and mycotoxin reduction
Oliveira P, et al.
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Articles

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Protocols

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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