D1946

DAF-FM DA

Name: DAF-FM DA
Brand: SIGMA
Price: 812 USD

≥98% (HPLC), NO detector, film

Synonym(s):

4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, Diaminofluorescein-FM diacetate

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₁₈F₂N₂O₇

CAS Number:

254109-22-3

Molecular Weight:

496.42

MDL number:

MFCD16872050

UNSPSC Code:

12352200

PubChem Substance ID:

329798717

NACRES:

NA.77

Key Documents

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Product Name

DAF-FM DA, ≥98% (HPLC)

Quality Level

100

assay

≥98% (HPLC)

form

film

storage condition

desiccated

color

colorless to yellow

fluorescence

λ_ex 500 nm; λ_em 515 nm in DMSO

shipped in

wet ice

storage temp.

−20°C

SMILES string

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChI key

BEVHTVRRVVEMEF-UHFFFAOYSA-N

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Show Differences

1 of 4

This Item	D1821	D2196	D2321
Sigma-Aldrich D1946 DAF-FM DA	Sigma-Aldrich D1821 DAF-FM	Sigma-Aldrich D2196 DAF-FM solution	Sigma-Aldrich D2321 DAF-FM DA solution
form film	form powder	form DMSO solution	form DMSO solution
assay ≥98% (HPLC)	assay ≥98% (HPLC)	assay ≥97% (HPLC)	assay ≥97% (HPLC)
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
storage condition desiccated	storage condition desiccated	storage condition desiccated	storage condition desiccated, protect from light
color colorless to yellow	color white to brown	color colorless to yellow, including pink	color clear colorless

Application

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates.[1] It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).[2][3]

Biochem/physiol Actions

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Features and Benefits

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Disclaimer

Hygroscopic

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Concentration-dependent osteogenic and angiogenic biological performances of calcium phosphate cement modified with copper ions

Zhang J, et al.

Materials Science and Engineering, C, 99, 1199-1212 (2019)

Nitric oxide signaling is involved in the response to iron deficiency in the woody plant Malus xiaojinensis.

Longmei Zhai et al.

Plant physiology and biochemistry : PPB, 109, 515-524 (2016-11-12)

To cope with iron (Fe) deficiency, plants have evolved a wide range of adaptive responses from changes in morphology to altered physiological responses. Recent studies have demonstrated that nitric oxide (NO) is involved in the Fe-deficiency response through hormonal signaling

The role of nitric oxide in the interaction of Arabidopsis thaliana with the biotrophic fungi, Golovinomyces orontii and Erysiphe pisi.

Markus Schlicht et al.

Frontiers in plant science, 4, 351-351 (2013-09-24)

Powdery mildews are a diverse group of pathogenic fungi that can infect a large number of plant species, including many economically important crops. However, basic and applied research on these devastating diseases has been hampered by the obligate biotrophic lifestyle

Iron uptake system mediates nitrate-facilitated cadmium accumulation in tomato (Solanum lycopersicum) plants.

Bing Fang Luo et al.

Journal of experimental botany, 63(8), 3127-3136 (2012-03-02)

Nitrogen (N) management is a promising agronomic strategy to minimize cadmium (Cd) contamination in crops. However, it is unclear how N affects Cd uptake by plants. Wild-type and iron uptake-inefficient tomato (Solanum lycopersicum) mutant (T3238fer) plants were grown in pH-buffered

Shear-stress mediated nitric oxide production within red blood cells: A dose-response.

Jarod T Horobin et al.

Clinical hemorheology and microcirculation, 71(2), 203-214 (2018-12-26)

Red blood cells (RBC) are exposed to varying shear stress while traversing the circulatory system; this shear initiates RBC-derived nitric oxide (NO) production. The current study investigated the effect of varying shear stress dose on RBC-derived NO production. Separated RBC

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