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D218

Sigma-Aldrich

Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse

clone 1A, buffered aqueous solution

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MDL number:
NACRES:
NA.41

conjugate

unconjugated

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1A, monoclonal

form

buffered aqueous solution

mol wt

antigen ~200 kDa

species reactivity

human (weakly), guinea pig, mouse, rat, rabbit

technique(s)

immunohistochemistry (frozen sections): 1:200
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1:500

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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D219A273A276
Gene Information

human ... CACNA1S(779)
mouse ... Cacna1s(12292)
rat ... Cacna1s(682930)

Gene Information

mouse ... Cacna2d1(12293)
rat ... Cacna2d1(25399)

Gene Information

human ... ATP1A3(478)
rat ... Atp1a3(24213)

Gene Information

human ... ATP1A1(476)

species reactivity

human (weakly), guinea pig, mouse, rat, rabbit

species reactivity

rat, mouse, rabbit, guinea pig

species reactivity

human, rat, monkey, sheep, rabbit, guinea pig, canine

species reactivity

canine, chicken, human, sheep, pig, bovine, mouse

clone

1A, monoclonal

clone

20A, monoclonal

clone

XVIF9-G10, monoclonal

clone

M7-PB-E9, monoclonal

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

mol wt

antigen ~200 kDa

mol wt

antigen 143 kDa (reduced), antigen 220 kDa (non-reduced)

mol wt

antigen ~110 kDa

mol wt

antigen ~110 kDa

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General description

DHP receptor is composed of at least four subunits. The α1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated Na+ channel. The α2 subunit is a large glycoprotein and the other 2 subunits are termed β and γ.

Specificity

Reacts specifically with the α1 subunit of the DHP receptor. May be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

Immunogen

rabbit muscle transverse tubule dihydropyridine (DHP) receptors.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse is suitable for immunoprecipitation, immunohistochemistry at a working dilution of 1:500 and immunoblotting at 1:2000. It was used for the detection and localization of DHPR-1α by western blotting in skeletal muscle tissue sections of mice in a study. The antibody may be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.

Biochem/physiol Actions

The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type Ca2+ channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Philippe Magown et al.
Nature communications, 6, 8506-8506 (2015-10-16)
Neural prostheses can restore meaningful function to paralysed muscles by electrically stimulating innervating motor axons, but fail when muscles are completely denervated, as seen in amyotrophic lateral sclerosis, or after a peripheral nerve or spinal cord injury. Here we show
F Galbiati et al.
The Journal of biological chemistry, 276(24), 21425-21433 (2001-03-22)
Caveolin-3, a muscle-specific caveolin-related protein, is the principal structural protein of caveolae membrane domains in striated muscle cells. Recently, we identified a novel autosomal dominant form of limb-girdle muscular dystrophy (LGMD-1C) in humans that is due to mutations within the
Bo Van et al.
FEBS letters, 589(10), 1095-1104 (2015-04-07)
Mitsugumin 56 (MG56), also known as the membrane-bound O-acyl-transferase family member hedgehog acyltransferase-like, was identified as a new sarcoplasmic reticulum component in striated muscle. Mg56-knockout mice grew normally for a week after birth, but shortly thereafter exhibited a suckling defect
Pankaj B Agrawal et al.
American journal of human genetics, 95(2), 218-226 (2014-08-05)
Centronuclear myopathies (CNMs) are characterized by muscle weakness and increased numbers of central nuclei within myofibers. X-linked myotubular myopathy, the most common severe form of CNM, is caused by mutations in MTM1, encoding myotubularin (MTM1), a lipid phosphatase. To increase

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