D225

Sigma-Aldrich

4,5-Diaminofluorescein diacetate solution

5 mM in DMSO, ≥97% (HPLC)

Synonym(s):
DAF-2 DA
MDL number:

Quality Level

assay

≥97% (HPLC)

availability

not available in Japan

concentration

5 mM in DMSO

shipped in

wet ice

storage temp.

−20°C

General description

4,5-Diaminofluorescein diacetate (DAF-2 DA) solution is a membrane-permeable fluorescent probe, which measures nitric oxide (NO) in living cells. It is used to study the synthesis of NO in bovine aortic endothelial cells (BAECs). DAF-2 DA imaging shows that ciliated epithelial cells produces high amount of NO than non-epithelial inflammatory cells.

Packaging

1 mg in glass bottle

Application

Cell-permeable fluorescent detector of nitric oxide in living cells.

Legal Information

DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874,590

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificate of Analysis
Certificate of Origin
Determinants of shear stress-stimulated endothelial nitric oxide production assessed in real-time by 4, 5-diaminofluorescein fluorescence
Qiu W, et al.
Biochemical and Biophysical Research Communications, 286(2), 328-335 (2001)
Increased nitric oxide production in nasal epithelial cells from allergic patients-RT-PCR analysis and direct imaging by a fluorescence indicator: DAF-2 DA
Takeno S, et al.
Clinical and Experimental Allergy, 31(6), 881-888 (2001)
Anne Marie W Bartosch et al.
Biophysical journal, 113(1), 101-108 (2017-07-13)
Nitric oxide (NO) is a regulatory molecule in the vascular system and its inhibition due to endothelial injury contributes to cardiovascular disease. The glycocalyx is a thin layer of glycolipids, glycoproteins, and proteoglycans on the surface of mammalian epithelial cells....
Rachel A Battaglia et al.
eLife, 8 (2019-11-05)
Alexander disease (AxD) is a fatal neurodegenerative disorder caused by mutations in glial fibrillary acidic protein (GFAP), which supports the structural integrity of astrocytes. Over 70 GFAP missense mutations cause AxD, but the mechanism linking different mutations to disease-relevant phenotypes...
Zoltán Serfőző et al.
Cellular signalling, 30, 67-81 (2016-11-26)
Olfaction, a chemosensory modality, plays a pivotal role in the orientation and behavior of invertebrates. The central olfactory processing unit in terrestrial stylomatophoran snails is the procerebrum, which contains NO synthesizing interneurons, whose oscillatory currents are believed to be the...
Articles
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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