meets EP testing specifications
meets USP testing specifications
2.7 (vs air)
0.42 mmHg ( 20 °C)
42 %, 63 °F
cell culture | hybridoma: suitable
n20/D 1.479 (lit.)
189 °C (lit.)
16-19 °C (lit.)
H2O: miscible (completely)
1.10 g/mL (lit.)
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DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
DMSO is usually used at 1-10%, depending on the cell line. For the cell freezing media that we offer, it is used at 8.7% concentration.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Product D2438 is assessed for cell freezing. Cells are frozen and stored at -70°C. The cells are thawed, cultured and viability is assessed at 24 hours. There is no evidence of cytotoxicity or abnormal cell growth.
DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
The industry standard for 'endotoxin free' is less than 1 EU/mL.
Products D1435 and D2438 both meet the USP specifications. The D2438 is sterile-filtered, but D1435 is not. Furthermore, the D2438 is quality control tested for endotoxin and use-tested for cell freezing.
DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.
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