2.7 (vs air)
0.42 mmHg ( 20 °C)
42 %, 63 °F
cell culture | hybridoma: suitable
n20/D 1.479 (lit.)
189 °C (lit.)
16-19 °C (lit.)
H2O: miscible (completely)
1.10 g/mL (lit.)
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DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
DMSO is usually used at 1-10%, depending on the cell line. For the cell freezing media that we offer, it is used at 8.7% concentration.
DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Product D2438 is assessed for cell freezing. Cells are frozen and stored at -70°C. The cells are thawed, cultured and viability is assessed at 24 hours. There is no evidence of cytotoxicity or abnormal cell growth.
DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
The industry standard for 'endotoxin free' is less than 1 EU/mL.
DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.
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