D2650

Dimethyl sulfoxide

Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

• Synonym(s): DMSO
• Linear Formula: (CH₃)₂SO
• CAS Number: 67-68-5
• Molecular Weight: 78.13
• Beilstein: 506008
• EC Number: 200-664-3
• MDL number: MFCD00002089
• PubChem Substance ID: 24893703
• NACRES: NA.75

Properties

• grade: Hybri-Max^™
• Quality Level: 400
• vapor density: 2.7 (vs air)
• vapor pressure: 0.42 mmHg ( 20 °C)
• sterility: sterile-filtered
• product line: BioReagent
• assay: ≥99.7%
• form: liquid
• autoignition temp.: 573 °F
• expl. lim.: 42 %, 63 °F
• technique(s): cell culture | hybridoma: suitable; cryopreservation: suitable
• impurities: endotoxin, tested; ≤0.1% water; water
• refractive index: n20/D 1.479 (lit.)
• bp: 189 °C (lit.)
• mp: 16-19 °C (lit.)
• solubility: H₂O: miscible (completely)
• density: 1.10 g/mL (lit.)
• SMILES string: CS(C)=O
• InChI: 1S/C2H6OS/c1-4(2)3/h1-2H3
• InChI key: IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Description

Packaging

5, 10, 100 mL

1 L

5 mL and 10 mL in flame sealed ampules, 100 mL in amber bottle

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves