D2812

Sigma-Aldrich

REDTaq® Genomic DNA Polymerase

without MgCl2

MDL number:
NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

form

liquid

feature

hotstart: no

concentration

1 unit/μL

color

red

Featured Industry

Agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

REDTaq Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The dye migrates slightly faster than bromophenol blue at approximately the same rate as a 125 base pair fragment.
The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.

Application

For routine genomic DNA amplifications

Features and Benefits

  • Enhanced amplification on genomic and difficult DNA templates
  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
  • Quick recognition and confirmation of appropriate mixing
  • No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel

Components

Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

Wiesław Bogdanowicz et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(30), 12279-12282 (2009-07-09)
We report the results of mitochondrial and nuclear DNA analyses of skeletal remains exhumed in 2005 at Frombork Cathedral in Poland, that are thought to be those of Nicolaus Copernicus (1473-1543). The analyzed bone remains were found close to the...
K L Mealey et al.
Journal of veterinary pharmacology and therapeutics, 38(5), 429-433 (2015-02-11)
The aim of this study was to sequence all exons of the ABCB1 (MDR1) gene in cats that had experienced adverse reactions to P-glycoprotein substrate drugs (phenotyped cats). Eight phenotyped cats were included in the study consisting of eight cats...
Protocols
Method for PCR using genomic DNA or other complex templates
Read More

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