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D2944

Sigma-Aldrich

Anti-D1 Dopamine Receptor antibody,Rat monoclonal

clone 1-1-F11 s.E6, purified from hybridoma cell culture

Synonym(s):
Anti-DRD1
MDL number:
NACRES:
NA.25

biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1-1-F11 s.E6, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, human, monkey

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using D1 dopamine receptor transfected HEK-293T cells
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... DRD1(1812)
rat ... Drd1a(24316)

General description

Monoclonal Anti-D1 Dopamine Receptor (rat IgG2a isotype) is derived from the rat hybridoma 1-1-F11 S.E6 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor. Dopamine receptors belong to the family of seven transmembrane domain G protein coupled receptors (GPCRs). Dopamine receptors are mainly localized in the striatum, limbic system, the brain cortex and the infundibulum. The D1-like family consists of the D1 and D5 receptors.

Immunogen

recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-D1 Dopamine Receptor antibody produced in rat has been used in:
  • immunohistochemistry
  • immunoblotting
  • immunocytochemistry
  • proximity ligation assay
  • co-immunoprecipitation
  • western blotting

Biochem/physiol Actions

D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. D1 receptors are expressed on airway smooth muscle (ASM) and regulate smooth muscle force via cAMP activation of protein kinase A. It is a potential target for therapeutic relaxation of ASM.

Target description

D1 Dopamine Receptor encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

Sold under exclusive license from Emory University

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Impact of early consumption of high-fat diet on the mesolimbic dopaminergic system
Naneix F, et al.
eNeuro, 4(3) (2017)
D1R/GluN1 complexes in the striatum integrate dopamine and glutamate signalling to control synaptic plasticity and cocaine-induced responses
Cahill E, et al.
Molecular Psychiatry, 19(12), 1295-1295 (2014)
Aliza T Ehrlich et al.
Molecular pharmacology, 84(3), 476-486 (2013-07-12)
The mechanism underlying the crosstalk between multiple G protein-coupled receptors remains poorly understood. We previously reported that prostaglandin E receptor EP1 facilitates dopamine D1 receptor signaling in striatal slices and promotes behavioral responses induced by D1 receptor agonists. Here, using
E Cahill et al.
Molecular psychiatry, 19(12), 1295-1304 (2014-07-30)
Convergent dopamine and glutamate signalling onto the extracellular signal-regulated kinase (ERK) pathway in medium spiny neurons (MSNs) of the striatum controls psychostimulant-initiated adaptive processes underlying long-lasting behavioural changes. We hypothesised that the physical proximity of dopamine D1 (D1R) and glutamate
A L Frederick et al.
Molecular psychiatry, 20(11), 1373-1385 (2015-01-07)
Hetero-oligomers of G-protein-coupled receptors have become the subject of intense investigation, because their purported potential to manifest signaling and pharmacological properties that differ from the component receptors makes them highly attractive for the development of more selective pharmacological treatments. In

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