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D3035

Sigma-Aldrich

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

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Synonym(s):
DNA
CAS Number:
MDL number:
NACRES:
NA.52

biological source

human placenta

Quality Level

grade

for molecular biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

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General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Application

Deoxyribonucleic acid from human placenta has been used:
  • to compare the efficiency of DNA quantification methods
  • as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
  • as an internal control to estimate the degree of fragmentation of circulating DNA
  • for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
  • to compare the 70-bp P5 exon sequence between DNA of different human and primate species
  • to calculate copy number of genes, relative to normal human tissue
  • in PCR reactions
For use in Southern hybridizations.

Recommended products

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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