D3035

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

• Synonym(s): DNA
• CAS Number: 9007-49-2
• MDL number: MFCD00130921
• NACRES: NA.52

Properties

• grade: for molecular biology
• Quality Level: 200
• form: buffered aqueous solution
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Application

Deoxyribonucleic acid from human placenta has been used:

• to compare the efficiency of DNA quantification methods
• as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
• as an internal control to estimate the degree of fragmentation of circulating DNA
• for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
• to compare the 70-bp P5 exon sequence between DNA of different human and primate species
• to calculate copy number of genes, relative to normal human tissue
• in PCR reactions

For use in Southern hybridizations.

Packaging

0.1, 0.5 mg

Recommended products

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves