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MilliporeSigma

D3687

Sigma-Aldrich

DirectLoad PCR 100 bp Low Ladder

ready-to-use marker for DNA electrophoresis

Synonym(s):

100bp ladder for gel electrophoresis, 100bp maker, DirectLoad marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

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About This Item

UNSPSC Code:
41105335
NACRES:
NA.31

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grade

Molecular Biology

Quality Level

form

liquid

usage

75 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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This Item
P1473D3937P9577
grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

75 uses

usage

75 uses

usage

100 uses

usage

sufficient for 50 uses

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

General description

Sigma′s DirectLoad PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.

Application

Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Components

Sigma′s DirectLoad PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

  1. How many mL are in one vial of the 100 bp ready to use DNA ladder?

    1 answer
    1. One vial of Product D3687, DirectLoad™ PCR 100 bp Low Ladder, contains 375 µL.

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