D3812

DirectLoad^™ 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

• Synonym(s): DirectLoad^™ 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad^™ 50 bp DNA Marker, Ready-to-use DirectLoad^™ 50 bp DNA Step Ladder
• UNSPSC Code: 41105335
• NACRES: NA.25

Properties

• Quality Level: 200
• form: (liquid)
• usage: sufficient for 100 loads
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

Description

General description

Sigma′s DirectLoad^™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

DirectLoad^™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.[1]

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

• Ready-to-load
• Easy-to-use
• Popular band sizes

Components

Sigma′s DirectLoad^™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable