Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

D4138

Deoxyribonuclease II from porcine spleen

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Synonym(s):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
20000 units

Available to ship TODAYfromMILWAUKEE

$66.00
80000 units

Available to ship TODAYfromMILWAUKEE

$218.00

About This Item

CAS Number:
9025-64-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
MFCD00130919
EC Number:
3.1.22.1 (BRENDA, IUBMB)
Specific activity:
2,000-6,000 Kunitz units/mg protein (biuret)

$66.00

Available to ship TODAYDetails

​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

type

Type IV

Quality Level

200

form

lyophilized powder

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

mol wt

45 kDa

composition

Protein, 60-80%

foreign activity

RNase ≤0.05%

storage temp.

−20°C

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase.[1] It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).[2]
Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions.[3] It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.[4]

Physical form

Contains sodium chloride

Other Notes

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
D8764D4513D4527
Deoxyribonuclease II from porcine spleen Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Sigma-Aldrich

D4138

Deoxyribonuclease II from porcine spleen

Deoxyribonuclease II from bovine spleen Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Sigma-Aldrich

D8764

Deoxyribonuclease II from bovine spleen

Deoxyribonuclease I from bovine pancreas Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Sigma-Aldrich

D4513

Deoxyribonuclease I from bovine pancreas

Deoxyribonuclease I from bovine pancreas Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Sigma-Aldrich

D4527

Deoxyribonuclease I from bovine pancreas

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

specific activity

≥1,000 units/mg protein

specific activity

≥2,000 units/mg protein

specific activity

≥2,000 units/mg protein

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

45 kDa

mol wt

38 kDa

mol wt

~31 kDa

mol wt

~31 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

foreign activity

RNase ≤0.05%

foreign activity

RNase ≤0.1%

foreign activity

Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

foreign activity

Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000535054
0000551098
0000551099
0000521164
0000521164

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

F Chianini et al.
Veterinary immunology and immunopathology, 82(3-4), 245-255 (2001-10-06)
The distribution of different cells of the immune system has been studied in formalin-fixed paraffin-embedded tissues from conventionally reared healthy pigs, using immunohistological techniques. The samples collected were: lungs, tonsils, lymph nodes (mediastinal, mesenteric, inguinal and submandibular), pancreas, spleen, liver
Yoko Kato-Toma et al.
The Biochemical journal, 371(Pt 1), 175-181 (2003-01-07)
13C-NMR spectroscopy was used to estimate the p K a values for the Tyr(150) (Y150) residue in wild-type and mutant class C beta-lactamases. The tyrosine residues of the wild-type and mutant lactamases were replaced with (13)C-labelled L-tyrosine ([ phenol -4-(13)C]tyrosine)
Lars Holmgren
Biochemical and biophysical research communications, 396(1), 147-151 (2010-05-25)
Horizontal or lateral gene transfer is an effective mechanism for the exchange of genetic information in bacteria allowing bacterial diversification and facilitating adaptation to new environments. Recent data demonstrate that DNA may also be transferred between somatic cells via the
View All Related Papers

Global Trade Item Number

SKUGTIN
D4138-20KU04061833564073
D4138-80KU04061832755441

Questions

Reviews

No rating value

Active Filters