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D4139

Sigma-Aldrich

N,N-Dimethyl-p-phenylenediamine dihydrochloride

suitable for peroxidase test, ≥99.0% (titration)

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Synonym(s):
4-Amino-N,N-dimethylaniline dihydrochloride, DMPD · 2HCl, DMPPDA · 2HCl
Linear Formula:
(CH3)2NC6H4NH2 · 2HCl
CAS Number:
Molecular Weight:
209.12
Beilstein:
3913463
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Assay

≥99.0% (titration)

form

powder

mp

222 °C (dec.) (lit.)

solubility

water: 50 mg/mL, clear

suitability

suitable for peroxidase test

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].Cl[H].CN(C)c1ccc(N)cc1

InChI

1S/C8H12N2.2ClH/c1-10(2)8-5-3-7(9)4-6-8;;/h3-6H,9H2,1-2H3;2*1H

InChI key

IAEDWDXMFDKWFU-UHFFFAOYSA-N

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This Item
07770193992186384
form

powder

form

powder

form

solid

form

solid

mp

222 °C (dec.) (lit.)

mp

210-215 °C (dec.), 222 °C (dec.) (lit.)

mp

34-36 °C (lit.)

mp

200-205 °C (dec.) (lit.)

solubility

water: 50 mg/mL, clear

solubility

-

solubility

-

solubility

water: soluble 50 mg/mL, clear to hazy, colorless to faintly yellow or pink

suitability

suitable for peroxidase test

suitability

-

suitability

-

suitability

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

food and beverages, microbiology

application(s)

-

application(s)

-

Application

N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) has been used in the HID-AB (high iron diamine-alcian blue) staining of intestinal tract samples.

Biochem/physiol Actions

N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) is used for the determination of peroxidase. DMPD is converted into a red pigment of semiquinone nature. It is also used in the HID-AB (high iron diamine-alcian blue) staining procedures, which are used to differentiate between sialomucin and sulfomucin in the gastrointestinal tract.

Caution

Darkens readily when exposed to air.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Customers Also Viewed

Colorimetric analysis with N, N-dimethyl-p-phenylenediamine of the uptake of intravenously injected horseradish peroxidase by various tissues of the rat.
STRAUS W
The Journal of Biophysical and Biochemical Cytology, 4, 541-541 (1958)
The distribution of mucous secreting cells in the gastrointestinal tracts of three small rodents from Saudi Arabia: Acomys dimidiatus, Meriones rex and Meriones libycus.
Johnson O, et al.
Acta Histochemica, 118, 118-118 (2016)
Digestive Disease Pathology (2013)
A method for serial tissue processing and parallel analysis of aberrant crypt morphology, mucin depletion, and Beta-catenin staining in an experimental model of colon carcinogenesis.
McGinley JN, et al.
Biological Procedures Online, 12, 9032-9032 (2010)
V Fogliano et al.
Journal of agricultural and food chemistry, 47(3), 1035-1040 (1999-12-20)
A novel method for measuring the antioxidant activity using N, N-dimethyl-p-phenylenediamine (DMPD) was developed. The radical cation of this compound gives a stable colored solution and a linear inhibition of color formation can be observed in the presence of 0.

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