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D4154

Sigma-Aldrich

Plasmid DNA from E. coli RRI

pUC18, buffered aqueous solution

CAS Number:
MDL number:
NACRES:
NA.85

Quality Level

grade

for molecular biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

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General description

Plasmid DNA from Escherichia coli RRI confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene. pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.

Application

Plasmid DNA from E. coli RRI has also been used for in vitro DNA transfection studies.
Plasmid DNA from Escherichia coli RRI has been used in polymerase chain reaction (PCR).

Biochem/physiol Actions

These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene; pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.
Foreign DNA inserted at the MCS abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. The orientations of the MCS regions in the pUC plasmids are analogous to those of the corresponding M13 phage.

Components

pUC18 Plasmid DNA, 10 μg is supplied at approximately 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Principle

Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Quantification of beta-human papillomavirus DNA by real-time PCR.
Weissenborn SJ
Nature Protocols, 5(1), 1-13 (2010)
Nucleoside phosphocholine amphiphile for in vitro DNA transfection
Moreau L, et al.
Molecular Biosystems, 1(3), 260-264 (2005)
Who owns human genes?: Is DNA patentable?
Lawrence O Gostin
JAMA, 310(8), 791-792 (2013-07-24)
Data disclosure crucial after DNA patent verdict.
Alex de Costa
Science (New York, N.Y.), 341(6149), 959-959 (2013-08-31)
Chao Liang et al.
Journal of nanoscience and nanotechnology, 13(6), 3999-4005 (2013-07-19)
LAMP is an isothermal amplification method that can achieve ultra-high sensitivity and specificity. However, the conventional detection of LAMP amplicons can lead to cross-contamination due to the need to open the reaction tube which contains a large number of amplicons.

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