D4184

Sigma-Aldrich

JumpStart Taq DNA Polymerase

without MgCl2

MDL number:
NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

form

liquid

feature

hotstart

concentration

2.5 units/μL

color

colorless

Featured Industry

Agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

JumpStart Taq DNA Polymerase (JumpStart Taq) is an optimized blend of Sigma′s high-performance Taq DNA Polymerase and JumpStart Taq antibody.

Application

JumpStart Taq DNA polymerase has been used:
  • In the PCR amplification of DNA isolated from herbarium specimens.
  • In the PCR reaction mixture for Randomly amplified polymorphic DNA polymerase chain reaction (RAPD PCR).
  • To amplify and detect a point mutation in the EGFR exon 19 using specific cancer cell lines.
  • In Real-time quantitative PCR
  • For PCR amplifications that require reduced non-specific amplification
  • For multiplex PCR
  • For reduction of primer dimers

Features and Benefits

  • Reduces non-specific amplification
  • Increases PCR specificity and yield
  • Reduces set-up time concerns associated with manual or wax Hot Start methods
  • Activation time of less than 1 minute

Packaging

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.
Supplied with 10× reaction buffer without MgCl2. Includes a separate tube of 25 mM MgCl2

Other Notes

Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Isabelle Desitter et al.
Anticancer research, 31(2), 427-441 (2011-03-08)
Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells. The ScreenCell® devices are single-use and low-cost innovative devices that use a filter...
I A Afonina et al.
BioTechniques, 32(4), 940-944 (2002-04-19)
Here we describe the properties of a novel class of oligonucleotide probes capable of sensitive hybridization-triggered fluorescence. These fluorogenic probes, known commercially as MGB Eclipse probes, are characterized by having a conjugated minor groove binder (MGB) ligand at the 5'-end...
Julia K Veir et al.
Veterinary therapeutics : research in applied veterinary medicine, 8(4), 229-238 (2008-01-10)
T evaluate the effect of supplementation with Enterococcus faecium strain SF68 (NCIMB10415) on immune function, responses to a multivalent vaccine were investigated in kittens given palatability enhancer with or without E. faecium SF68 daily. E. faecium SF68 was detected in...
James L Lordan et al.
Journal of immunology (Baltimore, Md. : 1950), 169(1), 407-414 (2002-06-22)
In sensitized individuals, exposure to allergens such as Dermatophagoides pteronyssinus (Der p) causes Th2 polarization and release of cytokines, including IL-4 and IL-13. Because Der p extracts also have direct effects on epithelial cells, we hypothesized that allergen augments the...
Julian R Starr et al.
Molecular ecology resources, 9 Suppl s1, 151-163 (2009-05-01)
We investigate the species discriminatory power of a subset of the proposed plant barcoding loci (matK, rbcL, rpoC1, rpoB, trnH-psbA) in Carex, a cosmopolitan genus that represents one of the three largest plant genera on earth (c. 2000 species). To...
Articles
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
Read More
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Read More
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Read More
Protocols
Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization
Read More
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Read More
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Read More

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