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Deoxyribonuclease I from bovine pancreas

Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Deoxyribonucleate 5′-oligonucleotido-hydrolase, DNase I
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

Quality Level




Type II-S


lyophilized powder

specific activity

≥2,000 units/mg protein

mol wt

~31 kDa

purified by



Protein, ≥80%


vial of ≥10.0 mg protein


endotoxin, tested


0.15 M NaCl: soluble 5.0 mg/mL, clear(lit.)


diagnostic assay manufacturing
diagnostic assay manufacturing

foreign activity

Chymotrypsin ≤0.01%
Protease ≤0.005%
RNase ≤0.01%

storage temp.


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Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the isolation and further characterization of carp liver DNase.
DNAse I from Sigma has been compared with human urine-derived interleukin 1 inhibitor for the ability to hydrolyze [14C]DNA [14C]DNA. It has also been used to cleave a 139 base pair Hind III/Nci I restriction fragment to investigate the stability of the enzyme for use in footprinting experiments. DNase I is widely used as a footprinting agent for studying drug and protein binding to DNA.
Used for the removal of DNA from protein samples.

Biochem/physiol Actions

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum lies between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Unit Definition

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. (1 unit = 1 Kunitz unit)

Physical form

Lyophilized powder containing calcium chloride

Preparation Note

The enzyme powder may be reconstituted in water or any buffer at pH 4.0-9.0, except phosphate buffer. Calcium chelators should be avoided. 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.

Analysis Note

Protein determined by biuret.


Health hazard

Signal Word


Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Enzymes of Molecular Biology
Weir, A. F.
Methods in Molecular Biology, 16 (1993)
D L Rosenstreich et al.
The Journal of experimental medicine, 168(5), 1767-1779 (1988-11-01)
We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been
Stability of DNase I in footprinting experiments.
B Ward et al.
Nucleic acids research, 16(17), 8724-8724 (1988-09-12)
Sambrook, J., and Russell, D.W.
Molecular Cloning: A Laboratory Manual, 2(2), 5-5 (2001)
Daniel Engelbertsen et al.
Scientific reports, 9(1), 10608-10608 (2019-07-25)
CD47, also known as integrin-associated protein (IAP), is a transmembrane protein with multiple biological functions including regulation of efferocytosis and leukocyte trafficking. In this study we investigated the effect of CD47-deficiency on atherosclerosis using a model of adeno-associated virus (AAV)-induced


Enzymatic Assay of Deoxyribonuclease I (EC

To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.

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