D4545

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer without MgCl₂

• Synonym(s): Taq polymerase, Taq polymerase enzyme
• CAS Number: 9012-90-2
• Enzyme Commission number: 2.7.7.7 (BRENDA, IUBMB)
• MDL number: MFCD00166026

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: liquid
• usage: sufficient for 1500 reactions; sufficient for 250 reactions; sufficient for 50 reactions; sufficient for 5000 reactions
• feature: dNTPs included: no; hotstart: no
• concentration: 5 units/μL
• technique(s): PCR: suitable
• color: colorless
• input: purified DNA
• suitability: suitable for PCR and automated sequencing reactions
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80°C) has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA.

Application

Taq DNA Polymerase from Thermus aquaticus has been used:

• in the process of DNA extraction (during gene amplification and sequencing)
• in genotyping
• in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8)
• for amplification of RNA from primary endothelial cells by conventional PCR

Packaging

50, 250, 250, 1500, 5000 units

Taq DNA Polymerase with 10× reaction buffer without MgCl₂. Includes a separate tube of 25 mM MgCl₂

Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl₂ (D1806) or a 10× reaction buffer without MgCl₂ plus a separate tube of MgCl₂ for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

• MgCl₂ provided in a separate tube to allow MgCl₂ optimization
• Can withstand repeated heating to 95 °C without significant loss of activity

Other Notes

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable