expressed in E. coli
sufficient for 1500 reactions
sufficient for 250 reactions
sufficient for 50 reactions
sufficient for 5000 reactions
dNTPs included: no
suitable for PCR and automated sequencing reactions
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12 - Non Combustible Liquids
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.