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D4645

Sigma-Aldrich

Sodium 3,5-dichloro-2-hydroxybenzenesulfonate

≥98% purity (TLC), powder

Synonym(s):

3,5-Dichloro-2-hydroxybenzenesulfonic acid, DCHBS, DHBS

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About This Item

Empirical Formula (Hill Notation):
C6H3Cl2NaO4S
CAS Number:
54970-72-8
Molecular Weight:
265.05
Beilstein/REAXYS Number:
3640643
EC Number:
259-416-8
MDL number:
MFCD00009798
UNSPSC Code:
12171500
PubChem Substance ID:
24893891
NACRES:
NA.47

Product Name

Sodium 3,5-dichloro-2-hydroxybenzenesulfonate, used for peroxide measurement

Quality Level

200

assay

≥98% (TLC)

form

powder

technique(s)

titration: suitable

color

white to off-white

pH

7 (20 °C, 53 g/L)

mp

>300 °C

solubility

H2O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Oc1c(Cl)cc(Cl)cc1S([O-])(=O)=O

InChI

1S/C6H4Cl2O4S.Na/c7-3-1-4(8)6(9)5(2-3)13(10,11)12;/h1-2,9H,(H,10,11,12);/q;+1/p-1

InChI key

NMWCVZCSJHJYFW-UHFFFAOYSA-M

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General description

Sodium 3,5-dichloro-2-hydroxybenzenesulfonate is used in conjunction with 4-aminoantipyrine (4-AAP) and hydrogen peroxide (H2O2) for chromogenic quantitation of uric acid in serum and urine samples.

Application

Used in conjunction with 4-aminoantipyrine (4-AAP) and hydrogen peroxide (H2O2) for chromogenic quantitation of peroxidase in coupled enzymatic reactions. Component of Trinder reagent for use with peroxidase to measure generation of hydrogen peroxide in automated systems.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCR1975
0000289388
0000237383
MKCT7831
MKCV4326

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Teresa Ramon-Marquez et al.
Talanta, 187, 83-90 (2018-06-02)
In this study, we have optimised the sterically directed attachment of biomolecules on the surface of coaxial membranes prepared by co-electrospinning which have been proved to be a material with very high performance for the development of biosensors with optical
Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymatic assay of uric acid in serum and urine.
Fossati P, et al.
Clinical Chemistry, 26(2), 227-231 (1980)
Lucas F de Castro et al.
Analytical and bioanalytical chemistry, 411(19), 4919-4928 (2019-04-04)
Microfluidic paper-based devices (μPADs) and wearable devices have been highly studied to be used as diagnostic tools due to their advantages such as simplicity and ability to provide instrument-free fast results. Diseases such as periodontitis and diabetes mellitus can potentially
J D Artiss et al.
Clinical chemistry, 30(8), 1389-1392 (1984-08-01)
A colorimetric peroxidase-coupled procedure for determination of creatinine in human serum and urine is described. A 30-s sample pre-treatment with bilirubin oxidase eliminates interference from endogenous bilirubin. The 4-aminoantipyrine-2-hydroxy-3,5-dichlorobenzenesulfonate chromogen system of this method is about fourfold more sensitive than
P Fossati et al.
Clinical chemistry, 29(8), 1494-1496 (1983-08-01)
We describe a new colorimetric method for measuring creatinine in serum and urine. Creatinine hydrolysis is catalyzed by creatinine amidohydrolase, and the creatine so produced is assayed in reactions catalyzed sequentially by creatine amidinohydrolase and sarcosine oxidase in a system
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