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  • D4692
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D4692

Sigma-Aldrich

Anti-DNMT1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-DNA methyltransferase
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~180 kDa

species reactivity

human, mouse

concentration

0.8-1.2 mg/mL

technique(s)

microarray: suitable
western blot: 0.5-1.0 μg/mL using nuclear extracts of 293T or HeLa cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... DNMT1(1786)

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SAB5701332SAB5700753D4567
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

-

conjugate

-

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

-

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

clone

polyclonal

clone

monoclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

liquid

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen ~180 kDa

mol wt

-

mol wt

144, 183, 184

mol wt

antigen ~180 kDa

General description

In humans, the gene encodes a DNA (cytosine-5)-methyltransferase with 1616 amino acids. The N-terminal contains the regulatory domain, while the C-terminal region contains the catalytic domain. Two isoforms of DNMT1 have been isolated, DNMT1a and DNMT1b, the difference residing in 16 extra amino acids within the latter.

Immunogen

synthetic peptide corresponding to amino acids 72-78 of human DNMT1, conjugated to KLH via an N-terminal added cysteine residue. The sequence is conserved in mouse and is different from the rat sequence by one amino acid.

Application

Anti-DNMT1 antibody produced in rabbit is suitable for microarray and immunoblotting at a working concentration of 0.5-1.0μg/mL using nuclear extracts of 293T or HeLa cells. It was used at 1:1000 working dilution for immunoblot analysis in a study to analyze whether some miRNAs are aberrantly expressed and target DNMT1 in NiS (nickel sulfide)-transformed cells. It was used for immunoprecipitation of DNMT1 from nuclear lysates of colon cancer cells transfected with DNMT1 vectors in a study. It was used as a primary antibody at a working dilution of 1:1000 for western blot analysis of total protein extracted from left murine testis tissue exposed to low-dose-rate radiation in a study.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

DNMT1 protein is important for the maintenance of methylation, as well as for the novo methylation activities occurring in somatic cells of vertebrates. It establishes a repressive transcription complex at replication foci with histone deacetylase HDAAC4 and DMAP1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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