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D4764

Sigma-Aldrich

Deoxyribonucleic acid from calf thymus

Genomic, unsheared

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Synonym(s):
double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
CAS Number:
91080-16-9
EC Number:
293-507-3
MDL number:
MFCD00151698
eCl@ss:
32160414
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

form

lyophilized powder

mol wt

>20 kb

storage temp.

2-8°C

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This Item
D4522D8899D8515
Sigma-Aldrich

Sigma-Aldrich

D4764

Deoxyribonucleic acid from calf thymus

Sigma-Aldrich

Sigma-Aldrich

D4522

Deoxyribonucleic acid from calf thymus

Sigma-Aldrich

Sigma-Aldrich

D8899

Deoxyribonucleic acid, single stranded from calf thymus

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA lyophilized powder

Sigma-Aldrich

D8515

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

>20 kb

mol wt

-

mol wt

~50 kb

mol wt

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Specificity

42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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