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D5167

Sigma-Aldrich

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse

clone 70.1, ascites fluid

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Synonym(s):
Anti-CILD1, Anti-DIC1, Anti-ICS1, Anti-PCD
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

70.1, monoclonal

contains

15 mM sodium azide

species reactivity

marsupial, hamster, human, Indian muntjac (Muntiacus muntjac vaginalis), chicken, rat, frog

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:2,000 using a chick brain extract

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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This Item
D1667V2258D2820
antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... DNAI1(27019), DNAI2(64446)
rat ... Dnai2(360654)

Gene Information

human ... DNAH1(25981), DNAH2(146754), DNAH3(55567), DNAH5(1767)

Gene Information

human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)

Gene Information

human ... DYNLT3(6990)
rat ... Dynlt3(363448)

species reactivity

marsupial, hamster, human, Indian muntjac (Muntiacus muntjac vaginalis), chicken, rat, frog

species reactivity

human, chicken

species reactivity

pig, mouse, sheep, human, bovine, rabbit, feline, rat

species reactivity

human, rat

clone

70.1, monoclonal

clone

440.4, monoclonal

clone

LN-6, monoclonal

clone

R1, monoclonal

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General description

Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus. Monoclonal anti-dynein (Intermediate Chain) antibody can be used for localization of dynein intermediate chain using different immunochemical assays like ELISA, immunoblotting and dot blot. Mouse anti- dynein (Intermediate Chain) antibody reacts specifically with dynein of frog, hamster (CHO cell line), chicken, rat, marsupial (rat kangaroo, PtK1 cell line), Indian muntjac (Muntiacus muntjac vaginalis) and human (HeLa,MCF-7).
Monoclonal anti-Dynein (Intermediate Chain) (mouse IgM isotype) is derived from the 70.1 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cytoplasmic dynein purified from chick brain. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (approximately 450 kDa), three intermediate chains (70- 125 kDa), and at least four light chains (15-25 kDa). Dynein intermediate chain (ICs) is a component of dynein multisubunit. Dynein intermediate chain interacts with dynactin through its N-terminal region.

Immunogen

cytoplasmic dynein from chicken embryo brain

Application

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse has been used in:
  • perturbation assays
  • western blotting
  • immunofluorescence microscopy
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunocytochemistry
  • immunopurification

Biochem/physiol Actions

Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Kinesin, Dynein, and Intracellular Transport.
Lodish H, Berk A, Zipursky SL, et al
Molecular and Cellular Biology, 4, Section 19-Section 19 (2000)
Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome
Casenghi M, et al.
Journal of Cell Science, 118(21), 5101-5108 (2005)
Eliza Zyłkiewicz et al.
The Journal of cell biology, 192(3), 433-445 (2011-02-02)
Ndel1 has been implicated in a variety of dynein-related processes, but its specific function is unclear. Here we describe an experimental approach to evaluate a role of Ndel1 in dynein-dependent microtubule self-organization using Ran-mediated asters in meiotic Xenopus egg extracts.
Mechanically distinct microtubule arrays determine the length and force response of the meiotic spindle
Takagi J, et al.
Developmental Cell, 49(2), 267-278 (2019)
Co-silencing of human Bub3 and dynein highlights an antagonistic relationship in regulating kinetochore-microtubule attachments
Silva P, et al.
Febs Letters, 589(23), 3588-3594 (2015)

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