If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
D5628
Digitonin
~50% (TLC)
Synonym(s):
Digitin
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$590.00
$2,300.00
$590.00
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InChI key
UVYVLBIGDKGWPX-KUAJCENISA-N
InChI
1S/C56H92O29/c1-19-7-10-56(75-17-19)20(2)31-45(85-56)37(67)32-22-6-5-21-11-26(24(61)12-55(21,4)23(22)8-9-54(31,32)3)76-50-42(72)39(69)44(30(16-60)80-50)81-53-48(47(36(66)29(15-59)79-53)83-49-40(70)33(63)25(62)18-74-49)84-52-43(73)46(35(65)28(14-58)78-52)82-51-41(71)38(68)34(64)27(13-57)77-51/h19-53,57-73H,5-18H2,1-4H3/t19-,20+,21+,22-,23+,24-,25-,26-,27-,28-,29-,30-,31+,32-,33+,34-,35+,36-,37+,38+,39-,40-,41-,42-,43-,44+,45-,46+,47+,48-,49+,50-,51+,52+,53+,54-,55+,56-/m1/s1
SMILES string
C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])[C@@H](O)[C@@]1([H])[C@@](CC[C@]5([H])[C@@]6(C[C@@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@H]7O[C@@](O[C@H](CO)[C@@H](O)[C@@H]8O[C@@](OC[C@@H](O)[C@@H]9O)([H])[C@@H]9O)([H])[C@@H]8O[C@@](O[C@H](CO)[C@H
biological source
plant seeds (Digitalis purpurea)
description
non-ionic
assay
50% (TLC)
form
powder
mol wt
micellar avg mol wt 70,000
concentration
~50% (TLC)
aggregation number
60
Quality Level
CMC
<0.5 mM (20-25°C)
mp
230-240 °C (dec.) (lit.)
solubility
ethanol: 10 mg/mL, clear, colorless to faintly yellow
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| form powder | form powder | form - | form - |
| assay 50% (TLC) | assay - | assay - | assay - |
| aggregation number 60 | aggregation number 60 | aggregation number - | aggregation number - |
| biological source plant seeds (Digitalis purpurea) | biological source plant seeds (Digitalis purpurea) | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level - |
| mol wt micellar avg mol wt 70,000 | mol wt micellar avg mol wt 70,000 | mol wt - | mol wt - |
General description
The detergent′s cellular permeabilization property extends to various cell types, enabling the study of intracellular components and processes by introducing antibodies, enzymes, or other molecules into cells. In immunocytochemistry experiments, Digitonin plays a crucial role by permeabilizing cells, allowing for the labeling and detection of intracellular proteins and structures, offering insights into cellular organization and protein localization. Furthermore, Digitonin facilitates RNA extraction and purification by lysing cells, a critical step for molecular biology applications such as gene expression analysis and RNA sequencing. The detergent′s utility is also evident in immunoprecipitation experiments, where it solubilizes membrane proteins, enabling the isolation of specific protein-antibody complexes for the study of protein interactions and signaling pathways.
Application
- to incubate isolated mitochondria for removing the mitochondrial outer membrane for mitoplasts preparation
- in the permeabilization of HeLa cells for in vitro import assay
- as a component in HEPES-buffered saline (HBS) to permeate the cells and release the aequorins for fluorescence and bioluminescence imaging
Biochem/physiol Actions
Features and Benefits
- Can extract membrane proteins like supercomplexes from the mitochondrial inner membrane
- Solubilizes 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) large unilamellar vesicles (LUVs) gradually at high temperature
- Canorm micelles at a wide range of concentrations
Other Notes
comparable product
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - STOT RE 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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