D5905

3,3′-Diaminobenzidine tetrahydrochloride

tablet, 10 mg substrate per tablet

• Synonym(s): 3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB
• Linear Formula: (NH₂)₂C₆H₃C₆H₃(NH₂)₂ · 4HCl
• Molecular Weight: 360.11
• Beilstein: 1212988
• EC Number: 231-018-9
• MDL number: MFCD00012969
• PubChem Substance ID: 24893981
• NACRES: NA.83

Properties

• Quality Level: 200
• form: tablet
• solubility: water: 1 tablet/15 mL (may contain small insoluble particles)
• storage temp.: −20°C
• SMILES string: Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
• InChI: 1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
• InChI key: KJDSORYAHBAGPP-UHFFFAOYSA-N

Description

Specificity

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Application

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

Reagent for spectrophotometric determination of selenium.

Biochem/physiol Actions

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Reconstitution

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H302 - H319 - H341 - H350
• Precautionary Statements: P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves