D5921

DMEM - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
• MDL number: MFCD00217342
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Product Name: Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: phenol red: no; sodium pyruvate: no; glucose: low; HEPES: no; L-glutamine: no; NaHCO₃: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.

Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:

• living colon cancer SW620 cells[1]
• human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231[2]
• mammal and canine derived adipose-derived mesenchymal stem cells[2]

Reconstitution

Supplement with 0.584 g/L L-glutamine.

Other Notes

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable