UN 1588 6.1 / PGII
This reagent cannot used for the determination of Hemoglobin in Matrigel plugs. This is because the sample being tested must be soluble so that it can be read in a spectrophotometer.
Reconstitute one vial of Drabkin's Reagent with 1000 ml of water. Then add 0.5 ml of the 30% Brij 35 Solution, Product Code B4184, to the 1000 ml of reconstituted Drabkin’s Reagent. Mix well and filter if insoluble particles remain.
The Hemoglobin standard concentrations of 60, 120, and 180 mg/mL listed in Column 4 of the table in the datasheet are corrected by the dilution factor of 1:251 (which is the dilution factor for 40 microliters of Hemoglobin Standard diluted in 10 mL of Drabkins's Reagent). This is done so that the user does not need to remember to multiply all calculated Hemoglobin concentrations in the unknowns being assayed by 251. The datasheet describes the preparation of a 180 mg/mL Cyanmethemoglobin Standard Solution. If you do not have an appropriate 180 mg/mL Cyanmethemoglobin Standard, prepare a 0.7171 mg/ml Cyanmethemoglobin Standard Solution by dissolving a suitable Hemoglobin in Drabkins's reagent. (c.f. Product No. H7379). Take any water content into account in weighing the Hemoglobin. Do not dilute this standard as described in step (2) of the datasheet.
Product No. H7379 Hemoglobin human can be used by dissolving in Drabkin's solution at a concentration of 0.7171 mg/ml. This will be equivalent to a 180 mg/ml Cyanmethemoglobin Standard Solution that is diluted 1:251 per our Datasheet. It is not necessary to use a Hemoglobin standard from the same species as is being tested.
This reagent shares the same formulation as the discontinued Sigma Diagnostics product 525-2 but it is intended only for research use, not for In Vitro Diagnostic use. The Diagnostic kit included a Hemoglobin standard that is no longer sold.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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