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MilliporeSigma

D6920

Sigma-Aldrich

2′-Deoxyadenosine 5′-triphosphate sodium salt solution

10 mM

Synonym(s):

dATP

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0.5 ML
$41.50

$41.50


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About This Item

EC Number:
UNSPSC Code:
41106305
NACRES:
NA.52

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assay

≥99%

Quality Level

form

liquid

concentration

10 mM

color

colorless

foreign activity

DNase, RNase, NICKase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3

InChI

1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1

InChI key

YJWCICGGRLOGEH-VWZUFWLJSA-M

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1 of 4

This Item
D478811051440001DATP-RO
Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

100

assay

≥99%

assay

≥99%

assay

98%

assay

99% (dNTP)

form

liquid

form

liquid

form

solution

form

solution

color

colorless

color

colorless

color

-

color

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

-

General description

2′-Deoxyadenosine 5′-triphosphate (dATP) is made up of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dATP is used by cells for synthesis of DNA by DNA polymerases.

Application

2′-Deoxyadenosine 5′-triphosphate sodium salt solution has been used as a component of the dNTP mix for labelling of ribosomal 18s rDNA probe and telomeric (TTAGG)n probe.[1]
2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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We herein describe the synthesis of fluorescent 5-(5,6-dimethoxybenzothiazol-2-yl)-2'-deoxyuridine 5'-triphosphate (d(bt)UTP) and primer extension reactions using d(bt)UTP. We also carried out primer extension reactions using the (bt)U template. B family DNA polymerases, such as KOD, Deep Vent (exo-), and 9°N(m) DNA

Protocols

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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