D7194

DAPK3, active, GST tagged human

Name: DAPK3, active, GST tagged human
Brand: SIGMA
Price: 433 USD

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

FLJ36473, ZIP

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.32

Key Documents

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recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

200

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

32-43 nmol/min·mg

mol wt

~79 kDa

UniProt accession no.

O43293

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... DAPK3(1613)

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This Item	D7069	SRP5018	K4268
Sigma-Aldrich D7194 DAPK3, active, GST tagged human	Sigma-Aldrich D7069 DAPK1 (1-363), active, GST tagged human	Sigma-Aldrich SRP5018 DAPK2, active, GST tagged human	Sigma-Aldrich K4268 PKCμ, active, GST tagged human
specific activity 32-43 nmol/min·mg	specific activity 42-57 nmol/min·mg	specific activity 115-156 nmol/min·mg	specific activity 578-782 nmol/min·mg
assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)
Gene Information human ... DAPK3(1613)	Gene Information human ... DAPK1(1612)	Gene Information human ... DAPK2(23604)	Gene Information human ... PRKD1(5587)
recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
mol wt ~79 kDa	mol wt ~71 kDa	mol wt ~67 kDa	mol wt ~131 kDa

Biochem/physiol Actions

DAPK3 or Death-associated protein kinase 3 (also known as ZIP) plays a role in apoptosis.DAPK3 is a nuclear serine/threonine-specific kinase that phosphorylates core histones H3 and H4, and myosine light chain in vitro. DAPK3 interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes. DAPK3 contains a leucine zipper structure at its C terminus and this region is responsible for binding to ATF4. The leucine zipper domain is necessary for the homodimerization of DAPK3 as well as for the activation of the kinase.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase.

Ute Preuss et al.

Nucleic acids research, 31(3), 878-885 (2003-02-01)

Death-associated protein (DAP)-like kinase (Dlk), also known as Zipper interacting protein (ZIP) kinase, is a nuclear serine/threonine-specific kinase that phosphorylates core histones H3 and H4, and myosine light chain in vitro. It interacts with transcription and splicing factors as well

Functional and Molecular Characterisation of Heart Failure Progression in Mice and the Role of Myosin Regulatory Light Chains in the Recovery of Cardiac Muscle Function.

Kasturi Markandran et al.

International journal of molecular sciences, 23(1) (2022-01-12)

Heart failure (HF) as a result of myocardial infarction (MI) is a major cause of fatality worldwide. However, the cause of cardiac dysfunction succeeding MI has not been elucidated at a sarcomeric level. Thus, studying the alterations within the sarcomere

ZIP kinase, a novel serine/threonine kinase which mediates apoptosis.

T Kawai et al.

Molecular and cellular biology, 18(3), 1642-1651 (1998-03-06)

We have identified a novel serine/threonine kinase, designated ZIP kinase, which mediates apoptosis. ZIP kinase contains a leucine zipper structure at its C terminus, in addition to a kinase domain at its N terminus. ZIP kinase physically binds to ATF4

The tumor suppressor kinase DAPK3 drives tumor-intrinsic immunity through the STING-IFN-β pathway.

Mariko Takahashi et al.

Nature immunology, 22(4), 485-496 (2021-03-27)

Evasion of host immunity is a hallmark of cancer; however, mechanisms linking oncogenic mutations and immune escape are incompletely understood. Through loss-of-function screening of 1,001 tumor suppressor genes, we identified death-associated protein kinase 3 (DAPK3) as a previously unrecognized driver

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