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D7291

Sigma-Aldrich

Deoxyribonuclease I RNase-free solution from bovine pancreas

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Synonym(s):
DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
CAS Number:
9003-98-9
Enzyme Commission number:
3.1.21.1 (BRENDA, IUBMB)
EC Number:
232-667-0
MDL number:
MFCD00130918
NACRES:
NA.53

biological source

bovine pancreas

Quality Level

200

grade

for molecular biology

form

buffered aqueous glycerol solution

specific activity

10000 units/mg protein





mol wt

29.1 kDa

UniProt accession no.

P00639

foreign activity

RNase, none detected

storage temp.

−20°C

Gene Information

cow ... DNASE1(282217)

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Sigma-Aldrich

Sigma-Aldrich

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

Deoxyribonuclease I from bovine pancreas Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Sigma-Aldrich

D4513

Deoxyribonuclease I from bovine pancreas

Millipore

Millipore

260913

DNase I, Bovine Pancreas

Deoxyribonuclease I from bovine pancreas Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Sigma-Aldrich

D4527

Deoxyribonuclease I from bovine pancreas

grade

for molecular biology

grade

-

grade

-

grade

-

form

buffered aqueous glycerol solution

form

lyophilized powder

form

lyophilized

form

lyophilized powder

specific activity

10000 units/mg protein





specific activity

≥2,000 units/mg protein

specific activity

≥60,000 dornase units/mg dry wt

specific activity

≥2,000 units/mg protein

mol wt

29.1 kDa

mol wt

~31 kDa

mol wt

-

mol wt

~31 kDa

UniProt accession no.

P00639

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

-

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Components

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

Unit Definition

One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Other Notes

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

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Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

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Protease from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG1

DNA Ligation Kit, null

AMPD1

DNase I, Amplification Grade

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Expression of anti-Mullerian hormone and its type II receptor in germ cells of maturing rat testis
Ohyama K, et al.
Endocrine Journal, EJ15-0370 (2015)
S Homburg et al.
The Journal of cell biology, 150(2), 293-307 (2000-07-26)
We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein
Stage-Specific Expression Profiles of Anti-Mullerian Hormone and its Type II Receptor in Germ Cells during Spermatogenic Cycle of Rats
Ohyama K, et al.
Journal of Steroids & Hormonal Science, 8(187), 2-2 (2017)
A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties
TAIT GCL and HARRIS WJ
European Journal of Biochemistry, 75(2), 357-364 (1977)
Umesh Kumar Dhawan et al.
Arteriosclerosis, thrombosis, and vascular biology, 41(10), 2598-2615 (2021-08-06)
Objective: Hypercholesterolemia-induced NETosis and accumulation of neutrophil extracellular traps (NETs) in the atherosclerotic lesion exacerbates inflammation and is causally implicated in plaque progression. We investigated whether hypercholesterolemia additionally impairs the clearance of NETs mediated by endonucleases such as DNase1 and
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Deoxyribonuclease I

Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.

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