D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

• Synonym(s): DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
• CAS Number: 9003-98-9
• EC Number: 3.1.21.1 (BRENDA, IUBMB)
• EC Number: 232-667-0
• MDL number: MFCD00130918
• UNSPSC Code: 12352204
• NACRES: NA.53

Properties

• biological source: bovine pancreas
• Quality Level: 200
• grade: Molecular Biology
• form: buffered aqueous glycerol solution
• specific activity: 10000 units/mg protein
• mol wt: 29.1 kDa
• concentration: 5—15 mg protein/mL
• technique(s): DNA extraction: suitable
• suitability: suitable for molecular biology
• UniProt accession no.: P00639
• application(s): cell analysis
• foreign activity: RNase, none detected
• storage temp.: −20°C
• Gene Information: cow ... DNASE1(282217)

Description

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent.[1] In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.[2][3][4]

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Other Notes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

One unit will cause a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)