D7808

Sigma-Aldrich

PCR Low Ladder Set

marker set for electrophoresis of PCR fragments

grade

for molecular biology

form

liquid

usage

50 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

2-8°C

General description

Sigma′s PCR PCR Low Ladder Set contains one vial each of the PCR 100 bp Low Marker and the PCR 20 bp Low Marker. When mixed together, the ladder appears with bands every 20 bp, with every 5th band more intense. About two ul of the mixed ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Components

Sigma′s PCR Low Ladder Set is provided as three separate tubes:
1 vial (25 ug) PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 vial (25 ug) PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 vial 6x loading buffer (250 ul volume) containing 15% Ficoll, 0.06% bromophenol blue, 0.06% xylene cyanol FF, and 30 mM EDTA.
1 additional empty tube is provided for storing the mixture.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.5%.

RIDADR

NONH for all modes of transport

Donatella Panatto et al.
Human vaccines & immunotherapeutics, 11(1), 270-276 (2014-12-09)
Infections caused by Chlamydia trachomatis (Ct) and human papillomavirus (HPV) are the two main sexually transmitted infections; however, epidemiological data on Ct prevalence and Ct/HPV co-infection in Italy are scant. This study aimed at estimating the prevalence of Ct infection...

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