As per our Enzymatic Assay of XYLANASE Activity in DRISELASE (EC 3.2.1.8):
Driselase (Xylanase) Enzyme Solution
(Immediately before use, prepare a solution containing 0.075 – 0.15 units/ml of Xylanase in
cold deionized water.) The pH information can also be found in the link below:
https://www.sigmaaldrich.cn/deepweb/assets/sigmaaldrich/product/documents/124/166/d9515enz.pdf
D8037
Driselase™ Basidiomycetes sp.
BioReagent, suitable for plant cell culture
Synonym(s):
Driselase™ from Basidiomycetes sp.
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product line
BioReagent
Quality Level
form
powder
composition
Protein, ≥10% biuret
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
−20°C
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This Item | D5417 | C1794 | D5912 |
|---|---|---|---|
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| form powder | form powder | form powder | form powder |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| product line BioReagent | product line BioReagent | product line BioReagent | product line BioReagent |
Application
Driselase™ Basidiomycetes sp. has been used:
- in spheroplast preparation from Coccomyxa cells
- in a CRISPR/Cas9-based mutagenesis protocol for Brachypodium distachyon and its allopolyploid relative, Brachypodium hybridum
- for cell wall digestion to perform whole-mount immunolocalization of Lotus japonicus root tissue
Biochem/physiol Actions
Driselase™ is a natural mixture of enzyme activities (fungal carbohydrates) used to digest plant cell walls to facilitate the maceration of plant materials, protoplast formation, and extraction processes. Driselase releases cell wall carbohydrates. This formulation contains enzyme activities of cellulose, endo-1,3-β-glucanase, and xylanase.
Other Notes
Crude powder containing laminarinase, xylanase and cellulase.
Legal Information
Driselase is a trademark of ASKA Animal Health Co. Ltd.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M C Ralet et al.
Carbohydrate research, 263(2), 227-241 (1994-10-17)
Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2
Dieter Hackenberg et al.
Plant physiology, 172(2), 1154-1166 (2016-08-24)
In this study, we report the functional characterization of heterotrimeric G-proteins from a nonvascular plant, the moss Physcomitrella patens. In plants, G-proteins have been characterized from only a few angiosperms to date, where their involvement has been shown during regulation
D T Kaplan et al.
Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes
T Ishii et al.
Carbohydrate research, 248, 179-190 (1993-10-04)
Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose
D P Blowers et al.
Plant physiology, 86(2), 505-509 (1988-02-01)
Plasma membrane vesicles from wild carrot cells grown in suspension culture were isolated by aqueous two-phase partitioning, and ATP-dependent phosphorylation was measured with [gamma-(32)P]ATP in the presence and absence of calcium. Treatment of the carrot cells with the cell wall
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Hi, do you know dissolvability and the optimum pH of this enzyme?
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