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MilliporeSigma

D8062

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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$55.80

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$291.00

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417

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Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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This Item
D8437D6421D8900
Sigma-Aldrich

Sigma-Aldrich

D8062

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

D8437

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D6421

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

D8900

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

powder

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

components

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

components

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

components

sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:
  • to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites[1]
  • to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment[2]
  • to culture mouse squamous cell carcinoma (SCCVII) tumor cells[3]

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL6949
RNBL5294
RNBL2018
RNBK9603
RNBK6544

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PeerJ, 6, e4187-e4187 (2018-01-10)
Since the development of human-induced pluripotent stem cells (hiPSCs), various types of hiPSC-derived cells have been established for regenerative medicine and drug development. Neural stem/progenitor cells (NSPCs) derived from hiPSCs (hiPSC-NSPCs) have shown benefits for regenerative therapy of the central
Tijmen H Booij et al.
SLAS discovery : advancing life sciences R & D, 22(8), 974-984 (2017-06-24)
Polycystic kidney disease (PKD) is a prevalent disorder characterized by renal cysts that lead to kidney failure. Various signaling pathways have been targeted to stop disease progression, but most interventions still focus on alleviating PKD-associated symptoms. The mechanistic complexity of
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Journal of medicinal chemistry, 61(12), 5269-5278 (2018-05-25)
Modulation of multiple biological targets with a single drug can lead to synergistic therapeutic effects and has been demonstrated to be essential for efficient treatment of CNS disorders. However, rational design of compounds that interact with several targets is very
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Neural interfaces for neuroscientific research are nowadays mainly manufactured using standard microsystems engineering technologies which are incompatible with the integration of carbon as electrode material. In this work, we investigate a new method to fabricate graphitic carbon electrode arrays on
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