D8187

Sigma-Aldrich

JumpStart REDTaq® DNA Polymerase

Hot-start Taq enzyme with inert dye, 10X buffer included

MDL number:
NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

form

liquid

feature

hotstart

concentration

1 unit/μL

color

red

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

JumpStart REDTaq DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.

  • The hot start Taq antibody prevents non-specific product formation.
  • Assembled PCR reactions can be placed at room temperature up to 2 hours.
  • Red tracer allows visible tracking of enzyme addition and reaction mixing.
  • PCR can be directly loaded onto an agarose gel without addition of loading buffers.
  • The red tracer is a tracking dye that run at 125bp on a 1% agarose gel.

Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.

Application

JumpStart REDTaq® DNA Polymerase has been used in:
  • polymerase chain reaction (PCR) for the amplification of insulin-enterotoxin ricin fusion gene (INS-RTB)
  • endothelial cells DNA derived from reverse transcribed RNA
  • leg genomic DNA from cricket flies

Features and Benefits

  • Prevents amplification of nonspecific products, resulting in increased efficiency and higher yields of the desired sequence
  • No lengthy activation step is required for enzyme activity to be restored
  • Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
  • Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye additions

Packaging

The enzyme is provided with an optimized 10× reaction buffer.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Other Notes

View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Target Organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Contributions of VEGF to age-dependent transmural gradients in contractile protein expression in ovine carotid arteries
Butler SM, et al.
American Journal of Physiology. Cell Physiology, 301(3), C653-C666 (2011)
Expression of a ricin toxin B subunit: insulin fusion protein in edible plant tissues
Carter JE, et al.
Molecular Biotechnology, 44(2), 90-100 (2010)
The field cricket Gryllus assimilis and two new sister species (Orthoptera: Gryllidae)
Weissman DB, et al.
Annals of the Entomological Society of America, 102(3), 367-380 (2009)
The field cricket Gryllus assimilis and two new sister species (Orthoptera: Gryllidae).
Weissman, D.B., et al.
Annals of the Entomological Society of America, 102, 367-367 (2009)
James E Carter et al.
Molecular biotechnology, 44(2), 90-100 (2009-11-10)
Onset of juvenile Type 1 diabetes (T1D) occurs when autoreactive lymphocytes progressively destroy the insulin-producing beta-cells in the pancreatic Islets of Langerhans. The increasing lack of insulin and subsequent onset of hyperglycemia results in increased damage to nerves, blood vessels...
Articles
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
Read More
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Read More
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Read More
Protocols
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Read More
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Read More
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Read More
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Read More

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