D8312

Sigma-Aldrich

REDTaq® Genomic DNA Polymerase

with MgCl2

MDL number:
NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

form

liquid

feature

hotstart: no

concentration

1 unit/μL

color

red

Featured Industry

Agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides (the building blocks of DNA). These enzymes are essential for DNA replication and usually work in pairs. It creates two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.
REDTaq Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The dye migrates slightly faster than bromophenol blue at approximately the same rate as a 125 base pair fragment.
The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.

Application

REDTaq® Genomic DNA Polymerase is used for routine PCR amplification from genomic DNA.

Features and Benefits

  • Enhanced amplification on genomic and difficult DNA templates
  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
  • Quick recognition and confirmation of appropriate mixing
  • No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel

Components

Includes 10× PCR Reaction Buffer

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Preparation of genomic DNA from Dictyostelium discoideum for PCR analysis.
Steve J Charette et al.
BioTechniques, 36(4), 574-575 (2004-04-20)
Gregory G Martin et al.
The Journal of biological chemistry, 278(24), 21429-21438 (2003-04-03)
Although liver fatty acid-binding protein (L-FABP) is an important binding site for various hydrophobic ligands in hepatocytes, its in vivo significance is not understood. We have therefore created L-FABP null mice and report here their initial analysis, focusing on the...
L M Winton et al.
Phytopathology, 92(1), 112-116 (2008-10-24)
ABSTRACT Phaeocryptopus gaeumannii is a widespread foliar parasite of Douglas-fir. Although normally innocuous, the fungus also causes the defoliating disease Swiss needle cast in heavily infected needles. The extent of P. gaeumannii colonization in Douglas-fir foliage was estimated with real-time...
Genotyping of HLA-B27 by real-time PCR without hybridization probes.
M A Bon et al.
Clinical chemistry, 46(7), 1000-1002 (2000-07-15)
Jill A Fahrner et al.
Cancer research, 62(24), 7213-7218 (2002-12-25)
We examined the relationship between aberrant DNA hypermethylation and key histone code components at a hypermethylated, silenced tumor suppressor gene promoter in human cancer. In lower eukaryotes, methylated H3-lysine 9 (methyl-H3-K9) determines DNA methylation and correlates with repressed gene transcription....
Protocols
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Read More
Method for PCR using genomic DNA or other complex templates
Read More

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