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D8406

Sigma-Aldrich

Monoclonal Anti-Dinitrophenyl antibody produced in mouse

IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-Dinitrophenyl, Anti Dnp Ige, Anti-DNP IGE - Monoclonal Anti-Dinitrophenyl antibody produced in mouse, Anti-Dnp Ige, Monoclonal Anti-DNP
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

SPE-7, monoclonal

form

buffered aqueous solution

concentration

~1 mg/mL

technique(s)

indirect ELISA: 0.05-0.1 μg/mL using using 0.5 μg/well DNP-BSA as the coating antigen and 1 μg/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody.

isotype

IgE

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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1 of 4

This Item
A2831SAB4200682A9044
conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

conjugate

-

conjugate

peroxidase conjugate

clone

SPE-7, monoclonal

clone

SPE-7, monoclonal

clone

GG-7, monoclonal

clone

polyclonal

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous solution

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General description

Dinitrophenyl (DNP) is a hapten that is often used for labeling primary or secondary probes in immunological assays. Hence, anti-DNP antibody is a useful tool for the detection and analysis of target molecules. Monoclonal Anti-DNP antibody is specific for DNP.
IgE gene is encoded on human chromosome 14. IgE is the least circulating antibody, but critical for eliciting hypersensitivity reaction against allergens.

Immunogen

DNP-KLH conjugate

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-Dinitrophenyl antibody is suitable for use in flow cytometry and immunocytochemistry.
Monoclonal antidinitrophenyl specific mouse IgE has been used in degranulation of rat mast cells using microplate reader and fluorescence spectrophotometer.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

Biochem/physiol Actions

IgE is produced by B cells by class switch recombination in response to allergens. IgE is also associated with mast cells and is abundantly produced in response to allergens especially in asthma and allergic rhinitis. IgE regulates mast cell homeostasis and is upregulated in food allergy. IgE is implicated in the pathogenesis of autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosus. IgE levels are modulated by other genes present on chromosome 14 especially in patients with asthma. Patients with mutations in signal transducer and activator of transcription 3 (STAT3) are associated high levels of IgE called the hyper–immunoglobulin E syndrome (HIES).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Linkage/association study of a locus modulating total serum IgE on chromosome 14q13?24 in families with asthma.
Mansur AH, et al.
Thorax, 59(10), 876-882 (2004)
Allergen drives class switching to IgE in the nasal mucosa in allergic rhinitis.
Takhar P, et al.
Journal of Immunology, 174(8), 5024-5032 (2005)
Role of IgE in autoimmunity.
Sanjuan MA, et al.
The Journal of Allergy and Clinical Immunology, 137(6), 1651-1661 (2016)
Beyond immediate hypersensitivity: evolving roles for IgE antibodies in immune homeostasis and allergic diseases.
Burton OT, et al.
Immunological Reviews, 242(1), 128-143 (2011)
Shenlu Qin et al.
Scientific reports, 6, 31320-31320 (2016-08-10)
Mast cells play an essential role in initiating allergic diseases. The activation of mast cells are controlled by a complicated signal network of reversible phosphorylation, and finding the key regulators involved in this network has been the focus of the

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