The deoxyribonucleic acid solution from calf thymus (item D8661) should be diluted with water.
D8661
Deoxyribonucleic acid solution from calf thymus
For hybridization
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$69.70
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biological source
bovine thymus
grade
Molecular Biology
assay
9-11 mg/mL (DNA concentration)
form
solution
shipped in
dry ice
storage temp.
−20°C
1 of 4
This Item | D4764 | D4522 | D8899 |
|---|---|---|---|
| biological source bovine thymus | biological source - | biological source - | biological source - |
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| assay 9-11 mg/mL (DNA concentration) | assay - | assay - | assay - |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
General description
A ready-to-use solution of high quality double-stranded template DNA isolated from the thymus of male and female calves. The solution is supplied at a concentration of 9 - 12 mg/ml .
Application
Deoxyribonucleic acid solution from calf thymus is suitable for use as a blocking agent in Southern hybridizations. It was used as negative control in DNA-DNA hybridization experiments using genomic DNA preparations of Vibrio parahaemolyticus and Vibrio alginolyticus.[1] It was used as a standard for DNA quantification by fluorescent assay.[2]
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Deoxyribonucleic acid solution from calf thymus has been used in in situ hybridization[3][4] and microarray hybridization.[2]
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Deoxyribonucleic acid solution from calf thymus has been used in in situ hybridization[3][4] and microarray hybridization.[2]
Sigma offers sonicated, denatured DNA as an aqueous solution, at a concentration of 10 mg per mL, for use as a blocking agent in Northern and Southern blotting.
Preparation Note
This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
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Annick Robert-Pillot et al.
FEMS microbiology letters, 215(1), 1-6 (2002-10-24)
We compared the efficiencies of biochemical methods and polymerase chain reaction (PCR) for the identification of Vibrio parahaemolyticus strains. The 122 isolates studied, identified by biochemical tests as V. parahaemolyticus or Vibrio alginolyticus, were tested by R72H PCR assay. The
Frequency of aneuploidy in in vitro-matured MII oocytes and corresponding first polar bodies in two dairy cattle (Bos taurus) breeds as determined by dual-color fluorescent in situ hybridization
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Theriogenology, 73(4), 523-529 (2010)
C Booth et al.
Nucleic acids research, 29(21), 4414-4422 (2001-11-03)
We have developed a method that allows quantitative amplification of single-stranded DNA (QAOS) in a sample that is primarily double-stranded DNA (dsDNA). Single-stranded DNA (ssDNA) is first captured by annealing a tagging primer at low temperature. Primer extension follows to
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What is the procedure for diluting item D8661, Deoxyribonucleic acid solution from calf thymus?
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