This formulation contains 100 mg/L of Magnesium Chloride Heptahydrate and 133 mg/L of Calcium Chloride Dihydrate. Please see the link below to review the full formulation of this and other Dulbecco's buffer products:
https://www.sigmaaldrich.com/product/sigma/d8662#product-documentation
D8662
Dulbecco’s Phosphate Buffered Saline
Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture
Dulbecco’s Phosphate Buffered Saline
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Synonym(s):
DPBS
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mL | Available to ship TODAYfromMILWAUKEE | $17.80 | |
| 500 mL | Available to ship TODAYfromMILWAUKEE | $33.40 | |
| 1 L | Available to ship TODAYfromMILWAUKEE | $54.80 | |
| 6 x 500 mL | Check Cart for Availability | $174.00 | |
| 6 x 1 L | Check Cart for Availability | $295.00 | |
| 24 x 500 mL | Check Cart for Availability | $627.00 |
About This Item
Product Name
Dulbecco′s Phosphate Buffered Saline, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
glucose: no (Dextro)
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.
Application
D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
- to wash cells for protein isolation
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR[1]
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells[2]
- to wash cells for protein isolation[2]
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
- provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
- serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
- when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
- provides a buffering system to maintain the medium within the physiological pH range
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. room temp | storage temp. room temp | storage temp. 2-8°C |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility 0.1 μm filtered |
| impurities endotoxin, tested | impurities endotoxin, tested | impurities - | impurities endotoxin, tested |
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
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E Valas Teuma et al.
Journal of cataract and refractive surgery, 45(8), 1177-1182 (2019-07-06)
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L Parmegiani et al.
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To study the efficacy and efficiency of a "universal warming protocol" for vitrified human embryos, based on subsequent steps with 1 and 0.5 M concentration of extracellular cryoprotectant (ECCP). Two studies on patients undergoing fertility treatments via ICSI: a prospective randomized
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8662-100ML | 04061838259417 |
| D8662-24X500ML | 04061833589786 |
| D8662-1L | 04061833589779 |
| D8662-500ML | 04061833589793 |
| D8662-6X1L | 04061833589809 |
| D8662-6X500ML | 04061833589816 |
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What is the concentration of MgCl2 and CaCl2?
1 answer-
Helpful?
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Can this solution be autoclaved?
1 answer-
This solution is already sterile-filtered and can be autoclaved at 121°C, 15 psi for 20 minutes.
Helpful?
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Could you give me the precise composition of this DPBS buffer solution (mixture of different salts)
1 answer-
The composition of this product, Dulbecco's Phosphate Buffered Saline, is available in the following Product Information Sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/137/207/d8662for.pdf
Helpful?
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¿Disponen de algún estudio de estabilidad de este producto a temperatura Ambiente? (15 - 30 ºC)
1 answer-
Any stability study information is considered proprietary and may not be shared. However, this sterile DPBS solution is quite stable. The product is assigned an expiration date of approximately 2 years from the date of quality release. The item ships at ambient temperature and is stable for up to two weeks to allow for transit time. For long term storage, refrigeration at 2-8°C is recommended.
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