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D8662

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
DPBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
glucose: no (Dextro)

shipped in

ambient

storage temp.

2-8°C

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This Item
D1408D1283D4031
form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

200

General description

Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.

Application

D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
  • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
  • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
  • to wash cells for protein isolation

Dulbecco′s Phosphate Buffered Saline has been used:
  • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
  • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
  • to wash cells for protein isolation

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
  • provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
  • serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
  • when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
  • provides a buffering system to maintain the medium within the physiological pH range

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

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