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D8899

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Synonym(s):

DNA single stranded

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52

grade

for molecular biology

Quality Level

form

lyophilized powder

mol wt

~50 kb

composition

ssDNA, ≥65%

storage temp.

−20°C

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General description

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Features and Benefits

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantity

One mg DNA is approx. 25 A260 units.

Preparation Note

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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J A Hansen et al.
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We evaluated the effects of recombinant porcine somatotropin (pST) and the beta-adrenergic agonist salbutamol on plasam endocrine and metabolite profiles and muscle chemistry in three genotypes of growing barrows (n = 96, 139 d old). Treatments were in a 2
H Kamei
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Eswaran Ramachandran et al.
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A series of four new palladium(II) complexes of 2-oxo-1,2-dihydroquinoline-3-carbaldehyde thiosemicarbazones with triphenylphosphine as coligand have been synthesized and characterized by the aid of various spectral techniques. The single-crystal X-ray diffraction studies revealed that the unsubstituted thiosemicarbazone ligand acted as monobasic

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