Upon reconstitution, the molarity of glucose is 17.5 mM (3.15 g/L).
D8900
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DMEM Hams F12, DME/F-12, 1:1 mixture
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 L | Check Cart for Availability | $124.00 | |
| 10 x 1 L | Check Cart for Availability | $132.00 | |
| 50 L | Check Cart for Availability | $529.00 |
About This Item
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:
- as a component of the culture medium for endometrial explants in vitro culture
- to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
- to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein
Preparation Note
Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.
1 of 1
This Item | |||
|---|---|---|---|
| form powder | form powder | form powder | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components sodium pyruvate: 0.055 g/L | components NaHCO3: no | components NaHCO3: no | components glucose: 3.15 g/L |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 400 |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
Nutrient Mixture F-12 Ham Formulation
High-quality ultrapure water that is endotoxin-free is recommended for mammalian cell culture as it support cell viability and prevents contamination.
Elena Ruggeri et al.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 23(6), 1197-1206 (2017-12-07)
Confocal microscopy was used to image stages of equine zygote development, at timed intervals, after intracytoplasmic sperm injection (ICSI) of oocytes that were matured in vivo or in vitro. After fixation for 4, 6, 8, 12, or 16 h after
Ryosuke Sakumoto et al.
Journal of animal science and biotechnology, 9, 46-46 (2018-06-28)
The aim of the present study was to evaluate CCL8 and CXCL10 expression and its regulatory mechanism in peripheral blood leukocytes (PBLs) at the time of maternal recognition in cows. Blood samples were collected on 14, 15, 16, 17 and
Sang Hwan Kim et al.
PeerJ, 7, e6344-e6344 (2019-02-08)
Here we investigated the expressions of apoptosis-associated genes known to induce programed cell death through mRNA expressions of two matrix metalloproteinases (MMPs) that are involved in the degradation of collagen and basal membrane in luteal cells cultured in the treatment
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8900-10X1L | 04061833590089 |
| D8900-50L | 04061835568376 |
| D8900-50K | 04061832954301 |
| D8900-10L | 04061833590072 |
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What is the molarity of glucose when reconstituted with water?
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