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  • D9156
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D9156

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from salmon testes

For hybridization

All Photos(1)
Synonym(s):
single-stranded template DNA
CAS Number:
68938-01-2
MDL number:
MFCD00130921
eCl@ss:
32160414
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

form

solution

concentration

10 mg/mL in H2O

shipped in

dry ice

storage temp.

−20°C

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D9156

Deoxyribonucleic acid, single stranded from salmon testes

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D7656

Deoxyribonucleic acid, single stranded from salmon testes

Deoxyribonucleic acid sodium salt from herring testes Type XIV

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D6898

Deoxyribonucleic acid sodium salt from herring testes

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D8661

Deoxyribonucleic acid solution from calf thymus

grade

for molecular biology

grade

for molecular biology

grade

-

grade

for molecular biology

form

solution

form

solution

form

solid

form

solution

concentration

10 mg/mL in H2O

concentration

10.0 ± 1.0 mg/mL in H2O

concentration

-

concentration

-

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

A ready-to-use solution of high quality single-stranded template DNA isolated from the testes of salmon. The solution is supplied at a concentration of 9 - 12 mg/ml .

Application

Deoxyribonucleic acid, single stranded from salmon testes is suitable for use as a blocking agent in Southern hybridizations. It was used for in situ hybridization histochemistry of bone marrow biopsy samples and human blood monocyte subpopulations.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Preparation Note

This DNA is ethanol precipitated and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.

Other Notes

DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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