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D9170

Sigma-Aldrich

Dimethyl sulfoxide

PCR Reagent

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Synonym(s):
DMSO
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein/REAXYS Number:
506008
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

PCR Reagent

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

assay

≥99.5%

form

liquid

autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

packaging

vial of 1 mL

technique(s)

PCR: suitable

impurities

≤0.1% water
water

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

suitability

suitable for PCR

application(s)

agriculture

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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This Item
D587934869D2438
Dimethyl sulfoxide PCR Reagent

D9170

Dimethyl sulfoxide

Dimethyl sulfoxide ReagentPlus®, ≥99.5%

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Dimethyl sulfoxide sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

D2438

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application(s)

agriculture

application(s)

-

application(s)

food and beverages

application(s)

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

400

assay

≥99.5%

assay

≥99.5%

assay

≥99.7%

assay

-

solubility

H2O: miscible (completely)

solubility

H2O: soluble(lit.), acetone: soluble(lit.), benzene: soluble(lit.), chloroform: soluble(lit.), ethanol: soluble(lit.)

solubility

-

solubility

H2O: miscible (completely)

form

liquid

form

liquid

form

liquid

form

liquid

General description

Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs). Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.

Application

Dimethyl sulfoxide has been used as a component of polymerase chain reaction (PCR) mixture and as PCR facilitator.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Microtitre Plate Based Cell-SELEX Method
Shorie M and Kaur H
Bio-protocol, 8(20) (2018)
Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials
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Applied Biochemistry and Biotechnology, 175(1), 3856-3862 (2015)
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Nature Protocols, 1(5), 2478-2478 (2006)
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, Todd C
Journal of Visualized Experiments, (63) (2012)
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and gamma-ray-irradiation, and freezing
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