All Photos(3)

D9307

Sigma-Aldrich

JumpStart Taq DNA Polymerase

with MgCl2

MDL number:

Quality Level

form

liquid

usage

sufficient for 1500 reactions
sufficient for 250 reactions
sufficient for 50 reactions

feature

dNTPs included: no
hotstart

concentration

2.5 units/μL

application(s)

PCR: suitable

color

colorless

input

purified DNA

Featured Industry

Agriculture

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Application

JumpStart Taq DNA Polymerase has been used in PCR (polymerase chain reaction) and to amplify DNA extracted from formaldehyde fixed paraffin embedded (FFPE) samples.
  • For PCR amplifications that require reduced non-specific amplification
  • For multiplex PCR
  • For reduction of primer dimers

Features and Benefits

  • Reduces non-specific amplification
  • Increases PCR specificity and yield
  • Reduces set-up time concerns associated with manual or wax Hot Start methods
  • Activation time of less than 1 minute

Packaging

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.
Supplied with 10× reaction buffer containing 15 mM MgCl2

Other Notes

Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

High quality bisulfite sequencing using nanogram amounts of genomic DNA
Sun J, et al.
International journal of biochemistry and biotechnology, 2, 449-456 (2013)
Yue Hu et al.
BMC genetics, 21(1), 112-112 (2020-09-23)
In order to study the relations of hepatocellular functions, weight gain and metabolic imbalance caused by low-dose antibiotics (LDA) via epigenetic regulation of gene transcription, 32 weaned piglets were employed as animal models and randomly allocated into two groups with...
Whole genome DNA methylation analysis based on high throughput sequencing technology
Li N, et al.
Methods, 52(3), 203-212 (2010)
Disruption of Abi1/Hssh3bp1 expression induces prostatic intraepithelial neoplasia in the conditional Abi1/Hssh3bp1 KO mice
Xiong X, et al.
Oncogenesis, 1(9), e26-e26 (2012)
Germán Gastón Leparc et al.
Nucleic acids research, 35(21), e146-e146 (2007-11-15)
One important goal of genomics is to explore the extent of alternative splicing in the transcriptome and generate a comprehensive catalog of splice forms. New computational and experimental approaches have led to an increase in the number of predicted alternatively...

Articles

Introduction and Historical Timelines

Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Protocols

Saliva DNA Extraction & WGA Amplification Protocol

Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Extraction & Amplification of whole blood using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.

Blood Card - Extraction & Amplification WGA Protocol

Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA has been extracted, it can then be amplified using the amplification protocol

See All

Related Content

Animal Tissue DNA-Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service