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D9307

Sigma-Aldrich

JumpStart Taq DNA Polymerase

with MgCl2

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Synonym(s):
hot start DNA polymerase, hot start PCR
MDL number:

Quality Level

form

liquid

usage

sufficient for 1500 reactions
sufficient for 250 reactions
sufficient for 50 reactions

feature

dNTPs included: no
hotstart

concentration

2.5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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This Item
D4184D5809D1313
concentration

2.5 units/μL

concentration

2.5 units/μL

concentration

2.5 unit/μL

concentration

1 unit/μL

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

color

colorless

color

colorless

color

colorless

color

red

suitability

suitable for PCR

suitability

suitable for PCR

suitability

suitable for PCR

suitability

suitable for PCR

application(s)

agriculture

application(s)

agriculture

application(s)

-

application(s)

agriculture

General description

JumpStart Taq DNA polymerase is a combination of Sigma′s high-performance Taq DNA Polymerase and JumpStart Taq antibody. The Taq DNA Polymerase activity is inactivated by combining the enzyme with JumpStart Taq antibody, a neutralizing monoclonal antibody to Taq DNA polymerase. Antibody inactivation provides a simple, efficient procedure for hot-start PCR. During PCR, JumpStart Taq DNA polymerase is inactive at low (room) temperature, as the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates, and the polymerase becomes fully active.

Application

JumpStart Taq DNA Polymerase has been used:
  • in the amplification of DNA libraries of varying sizes
  • in a methylation-specific, quantitative real-time polymerase chain reaction (MS-qPCR) to determine the BRCA1 promoter methylation status
  • in the generation of plasmid by amplifying the full-length of HIF1β via PCR
  • For PCR amplifications that require reduced non-specific amplification
  • For multiplex PCR
  • For reduction of primer dimers

Features and Benefits

  • Reduces non-specific amplification
  • Increases PCR specificity and yield
  • Reduces set-up time concerns associated with manual or wax Hot Start methods
  • Activation time of less than 1 minute

Packaging

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.
Supplied with 10× reaction buffer containing 15 mM MgCl2

Other Notes

Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Germán Gastón Leparc et al.
Nucleic acids research, 35(21), e146-e146 (2007-11-15)
One important goal of genomics is to explore the extent of alternative splicing in the transcriptome and generate a comprehensive catalog of splice forms. New computational and experimental approaches have led to an increase in the number of predicted alternatively
Comparison of anthracnose resistance with the presence of two SCAR markers associated with the Rca2 gene in strawberry.
Miller-Butler MA, et al.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 793- 798 (2019)
Seohee Chang et al.
Biomolecules, 10(4) (2020-04-03)
Phage display is one of the most frequently used platform technologies utilized to screen and select therapeutic antibodies, and has contributed to the development of more than 10 therapeutic antibodies used in the clinic. Despite advantages like efficiency and low
Masakazu Kamata et al.
Human gene therapy, 21(11), 1555-1567 (2010-06-08)
The recent development of induced pluripotent stem cells (iPSCs) by ectopic expression of defined reprogramming factors offers enormous therapeutic opportunity. To deliver these factors, murine leukemia virus (MLV)-based vectors have been broadly used in the setting of hematopoietic stem cell
Yue Hu et al.
BMC genetics, 21(1), 112-112 (2020-09-23)
In order to study the relations of hepatocellular functions, weight gain and metabolic imbalance caused by low-dose antibiotics (LDA) via epigenetic regulation of gene transcription, 32 weaned piglets were employed as animal models and randomly allocated into two groups with

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