All Photos(3)
Quality Level
form
liquid
feature
hotstart
concentration
2.5 units/μL
color
colorless
Featured Industry
Agriculture
shipped in
wet ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
Related Categories
Application
JumpStart™ Taq DNA Polymerase has been used in PCR (polymerase chain reaction) and to amplify DNA extracted from formaldehyde fixed paraffin embedded (FFPE) samples.
- For PCR amplifications that require reduced non-specific amplification
- For multiplex PCR
- For reduction of primer dimers
Features and Benefits
- Reduces non-specific amplification
- Increases PCR specificity and yield
- Reduces set-up time concerns associated with manual or wax Hot Start methods
- Activation time of less than 1 minute
Packaging
JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl2 for optimization.
Supplied with 10× reaction buffer containing 15 mM MgCl2
Other Notes
Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
RIDADR
NONH for all modes of transport
WGK Germany
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
Certificate of Origin
Sebastian Thams et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 27(1), 87-101 (2018-11-18)
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease selectively targeting motor neurons in the brain and spinal cord. The reasons for differential motor neuron susceptibility remain elusive. We developed a stem cell-based motor neuron assay to study cell-autonomous mechanisms causing...
X Xiong et al.
Oncogenesis, 1, e26-e26 (2012-01-01)
Prostate cancer is one of the leading causes of cancer-related deaths in the United States and a leading diagnosed non-skin cancer in American men. Genetic mutations underlying prostate tumorigenesis include alterations of tumor suppressor genes. We tested the tumor suppressor...
High quality bisulfite sequencing using nanogram amounts of genomic DNA
Sun J, et al.
International journal of biochemistry and biotechnology, 2, 449-456 (2013)
Yue Hu et al.
BMC genetics, 21(1), 112-112 (2020-09-23)
In order to study the relations of hepatocellular functions, weight gain and metabolic imbalance caused by low-dose antibiotics (LDA) via epigenetic regulation of gene transcription, 32 weaned piglets were employed as animal models and randomly allocated into two groups with...
Whole genome DNA methylation analysis based on high throughput sequencing technology
Li N, et al.
Methods, 52(3), 203-212 (2010)
Articles
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
Read MoreProtocols
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Read MoreMycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
Read MoreWhole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.
Read MoreBlood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA has been extracted, it can then be amplified using the amplification protocol
Read MoreRelated Content
GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.
Read MoreOur team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical ServiceSupport
Customer SupportContact UsFAQSafety Data Sheets (SDS)Certificates (COA/COO)Quality & RegulatoryCalculators & AppsWebinarsSocial Media
MilliporeSigma
Research. Development. Production.
We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.
© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
Reproduction of any materials from the site is strictly forbidden without permission.