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D9663

Sigma-Aldrich

Donkey serum

Synonym(s):

Serum

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10 ML
$47.70

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10 ML
$47.70

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
biological source:
donkey
sterility:
non-sterile
shipped in:
dry ice

$47.70


In StockDetails


Request a Bulk Order

biological source

donkey

Quality Level

sterility

non-sterile

contains

preservative

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

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1 of 4

This Item
G9023566460R9133
Goat serum

G9023

Goat serum

biological source

donkey

biological source

goat

biological source

-

biological source

rabbit

sterility

non-sterile

sterility

non-sterile

sterility

-

sterility

non-sterile

application(s)

research pathology

application(s)

research pathology

application(s)

-

application(s)

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

contains

preservative

contains

preservative

contains

-

contains

preservative

General description

Donkey serum is pooled from a normal donor herd. In immunoassays, normal donkey serum can serve as a blocking agent and a control. It is used as a blocking agent in various studies for immunohistochemistry and immunocytochemistry, ranging from 5% to 20%.[1]

Application

Donkey serum has been used:
  • as a component in blocking buffer for immunofluorescence[2]
  • in phosphate buffer saline (PBS) as a blocking agent[2]
  • as a blocking agent in frozen immunocytochemistry and immunohistochemistry[3]
  • to prevent nonspecific binding in parvalbumin immunohistochemistry[4]

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

1–3 of 3 Questions  
  1. where can I find the protocol to reconstitute this donkey serum

    1 answer
    1. A protocol is not associated with this product. A preservative is added to this serum, making it unsuitable for cell culture. However, in blocking applications, it is typically used at a concentration of 5% - 20%. It may be helpful to view the 'Peer Reviewed Papers' and 'Protocols and Articles' section of the product detail page.

      Helpful?

  2. Is heat inactivation required for D9663 in immunofluorescence (IF)? I found information stating that it is not necessary for immunohistochemistry (IHC). If this applies to IF as well, what is the reason behind it?

    1 answer
    1. Donkey serum does not require heat inactivation for immunofluorescence. It is important to note that this is not a sterile product. While the lab may have routinely heat inactivated their serums for immunofluorescence, this is likely for worker safety rather than a specific requirement for performing immunofluorescence.

      Helpful?

  3. Does donkey serum D9663-10ML require heat inactivation prior to immunohistochemical assay?

    1 answer
    1. There is no need to heat inactivate donkey serum for blocking purposes.

      Helpful?

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