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DMN10

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GenElute Direct mRNA Miniprep Kits

sufficient for 10 purifications

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Synonym(s):
GenElute Direct mRNA Miniprep Kit, GenElute mRNA Kit, Gen Elute
NACRES:
NA.52

usage

sufficient for 10 purifications

Quality Level

technique(s)

RNA purification: suitable

storage temp.

15-25°C

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This Item
MRN70DMN70MRN10
Sigma-Aldrich

MRN70

GenElute mRNA Miniprep Kit

Sigma-Aldrich

MRN10

GenElute mRNA Miniprep Kit

usage

sufficient for 10 purifications

usage

sufficient for 70 purifications

usage

sufficient for 70 purifications

usage

sufficient for 10 purifications

DMN70

MRN10

DMN10

MRN70

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The GenElute Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 107 mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Application

The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Features and Benefits

  • mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
  • Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
  • Oligo(dT) polystyrene beads require fewer wash steps

Other Notes

Use for isolating mRNA directly from mammalian cells or tissues.
For additional information, please see www.sigma-aldrich.com/mrna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description

  • Elution solution 1.5 mL

  • Filtration columns with tubes 10 ea

  • Lysis solution 20 mL

  • 5 M NaCl 1.5 mL

  • Oligo(dT)-polystyrene beads .3 mL

  • Proteinase K 5 mg

  • 40% Glycerol solution .6 mL

  • Spin columns with tubes 10 ea

  • Collection tube 10 ea

See All (9)

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Brahmchetna Singh et al.
Molecular cancer, 6, 82-82 (2007-12-26)
Retinoids are potent growth inhibitory and differentiating agents in a variety of cancer cell types. We have shown that retinoids induce growth arrest in all pancreatic cancer cell lines studied, regardless of their p53 and differentiation status. However, the mechanism
Abhirami Visvanathan et al.
Genes, 10(2) (2019-02-20)
Despite recent advances in N⁶-methyladenosine (m⁶A) biology, the regulation of crucial RNA processing steps by the RNA methyltransferase-like 3 (METTL3) in glioma stem-like cells (GSCs) remains obscure. An integrated analysis of m⁶A-RIP (RNA immunoprecipitation) and total RNA-Seq of METTL3-silenced GSCs

Protocols

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

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